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Bag, I., Dale, R.K., Palmer, C., Lei, E.P. (2019). The zinc-finger protein CLAMP promotes gypsy chromatin insulator function in Drosophila.  J. Cell Sci. 132(5): jcs226092.
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Research paper

Chromatin insulators are DNA-protein complexes that establish independent higher-order DNA domains to influence transcription. Insulators are functionally defined by two properties: they can block communication between an enhancer and a promoter, and also act as a barrier between heterochromatin and euchromatin. In Drosophila, the gypsy insulator complex contains three core components; Su(Hw), CP190 and Mod(mdg4)67.2. Here, we identify a novel role for Chromatin-linked adaptor for MSL proteins (CLAMP) in promoting gypsy chromatin insulator function. When clamp is knocked down, gypsy-dependent enhancer-blocking and barrier activities are strongly reduced. CLAMP associates physically with the core gypsy insulator complex, and ChIP-seq analysis reveals extensive overlap, particularly with promoter-bound CP190 on chromatin. Depletion of CLAMP disrupts CP190 binding at a minority of shared sites, whereas depletion of CP190 results in extensive loss of CLAMP chromatin association. Finally, reduction of CLAMP disrupts CP190 localization within the nucleus. Our results support a positive functional relationship between CLAMP and CP190 to promote gypsy chromatin insulator activity.

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PMC6432716 (PMC) (EuropePMC)
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Correction: The zinc-finger protein CLAMP promotes gypsy chromatin insulator function in Drosophila (doi:10.1242/jcs.226092).
Bag et al., 2019, J. Cell Sci. 132(6): jcs231977 [FBrf0241914]

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    J. Cell Sci.
    Journal of Cell Science
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