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Shokri, L., Inukai, S., Hafner, A., Weinand, K., Hens, K., Vedenko, A., Gisselbrecht, S.S., Dainese, R., Bischof, J., Furger, E., Feuz, J.D., Basler, K., Deplancke, B., Bulyk, M.L. (2019). A Comprehensive Drosophila melanogaster Transcription Factor Interactome.  Cell Rep. 27(3): 955--970.e7.
FlyBase ID
FBrf0241977
Publication Type
Research paper
Abstract
Combinatorial interactions among transcription factors (TFs) play essential roles in generating gene expression specificity and diversity in metazoans. Using yeast 2-hybrid (Y2H) assays on nearly all sequence-specific Drosophila TFs, we identified 1,983 protein-protein interactions (PPIs), more than doubling the number of currently known PPIs among Drosophila TFs. For quality assessment, we validated a subset of our interactions using MITOMI and bimolecular fluorescence complementation assays. We combined our interactome with prior PPI data to generate an integrated Drosophila TF-TF binary interaction network. Our analysis of ChIP-seq data, integrating PPI and gene expression information, uncovered different modes by which interacting TFs are recruited to DNA. We further demonstrate the utility of our Drosophila interactome in shedding light on human TF-TF interactions. This study reveals how TFs interact to bind regulatory elements in vivo and serves as a resource of Drosophila TF-TF binary PPIs for understanding tissue-specific gene regulation.
PubMed ID
PubMed Central ID
PMC6485956 (PMC) (EuropePMC)
Related Publication(s)
Personal communication to FlyBase
Y2H bait-prey info for Drosophila transcription factor interactome.
Bulyk, 2019.5.3, Y2H bait-prey info for Drosophila transcription factor interactome. [FBrf0242401]
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Cell Rep.
    Title
    Cell reports
    ISBN/ISSN
    2211-1247
    Data From Reference
    Genes (585)
    Physical Interactions (2088)
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