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Citation
Berson, A., Goodman, L.D., Sartoris, A.N., Otte, C.G., Aykit, J.A., Lee, V.M., Trojanowski, J.Q., Bonini, N.M. (2019). Drosophila Ref1/ALYREF regulates transcription and toxicity associated with ALS/FTD disease etiologies.  Acta Neuropathol. Commun. 7(1): 65.
FlyBase ID
FBrf0242189
Publication Type
Research paper
Abstract

RNA-binding proteins (RBPs) are associated with amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), but the underlying disease mechanisms remain unclear. In an unbiased screen in Drosophila for RBPs that genetically interact with TDP-43, we found that downregulation of the mRNA export factor Ref1 (fly orthologue to human ALYREF) mitigated TDP-43 induced toxicity. Further, Ref1 depletion also reduced toxicity caused by expression of the C9orf72 GGGGCC repeat expansion. Ref1 knockdown lowered the mRNA levels for these related disease genes and reduced the encoded proteins with no effect on a wild-type Tau disease transgene or a control transgene. Interestingly, expression of TDP-43 or the GGGGCC repeat expansion increased endogenous Ref1 mRNA levels in the fly brain. Further, the human orthologue ALYREF was upregulated by immunohistochemistry in ALS motor neurons, with the strongest upregulation occurring in ALS cases harboring the GGGGCC expansion in C9orf72. These data support ALYREF as a contributor to ALS/FTD and highlight its downregulation as a potential therapeutic target that may affect co-existing disease etiologies.

PubMed ID
PubMed Central ID
PMC6487524 (PMC) (EuropePMC)
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Acta Neuropathol. Commun.
    Title
    Acta neuropathologica communications
    ISBN/ISSN
    2051-5960
    Data From Reference