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Citation
Zhao, F., Zhu, J.Y., Richman, A., Fu, Y., Huang, W., Chen, N., Pan, X., Yi, C., Ding, X., Wang, S., Wang, P., Nie, X., Huang, J., Yang, Y., Yu, Z., Han, Z. (2019). Mutations in NUP160 Are Implicated in Steroid-Resistant Nephrotic Syndrome.  J. Am. Soc. Nephrol. 30(5): 840--853.
FlyBase ID
FBrf0242226
Publication Type
Research paper
Abstract

Studies have identified mutations in >50 genes that can lead to monogenic steroid-resistant nephrotic syndrome (SRNS). The NUP160 gene, which encodes one of the protein components of the nuclear pore complex nucleoporin 160 kD (Nup160), is expressed in both human and mouse kidney cells. Knockdown of NUP160 impairs mouse podocytes in cell culture. Recently, siblings with SRNS and proteinuria in a nonconsanguineous family were found to carry compound-heterozygous mutations in NUP160. We identified NUP160 mutations by whole-exome and Sanger sequencing of genomic DNA from a young girl with familial SRNS and FSGS who did not carry mutations in other genes known to be associated with SRNS. We performed in vivo functional validation studies on the NUP160 mutations using a Drosophila model. We identified two compound-heterozygous NUP160 mutations, NUP160R1173× and NUP160E803K . We showed that silencing of Drosophila NUP160 specifically in nephrocytes (fly renal cells) led to functional abnormalities, reduced cell size and nuclear volume, and disorganized nuclear membrane structure. These defects were completely rescued by expression of the wild-type human NUP160 gene in nephrocytes. By contrast, expression of the NUP160 mutant allele NUP160R1173× completely failed to rescue nephrocyte phenotypes, and mutant allele NUP160E803K rescued only nuclear pore complex and nuclear lamin localization defects. Mutations in NUP160 are implicated in SRNS. Our findings indicate that NUP160 should be included in the SRNS diagnostic gene panel to identify additional patients with SRNS and homozygous or compound-heterozygous NUP160 mutations and further strengthen the evidence that NUP160 mutations can cause SRNS.

PubMed ID
PubMed Central ID
PMC6493979 (PMC) (EuropePMC)
Related Publication(s)
Note

Filling the Gap: Drosophila Nephrocytes as Model System in Kidney Research.
Marelja and Simons, 2019, J. Am. Soc. Nephrol. 30(5): 719--720 [FBrf0242293]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    J. Am. Soc. Nephrol.
    Title
    Journal of the American Society of Nephrology
    Publication Year
    1990-
    ISBN/ISSN
    1046-6673
    Data From Reference
    Alleles (6)
    Genes (4)
    Human Disease Models (1)
    Natural transposons (1)
    Experimental Tools (2)
    Transgenic Constructs (6)