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Citation
Fingerhut, J.M., Moran, J.V., Yamashita, Y.M. (2019). Satellite DNA-containing gigantic introns in a unique gene expression program during Drosophila spermatogenesis.  PLoS Genet. 15(5): e1008028.
FlyBase ID
FBrf0242308
Publication Type
Research paper
Abstract

Intron gigantism, where genes contain megabase-sized introns, is observed across species, yet little is known about its purpose or regulation. Here we identify a unique gene expression program utilized for the proper expression of genes with intron gigantism. We find that two Drosophila genes with intron gigantism, kl-3 and kl-5, are transcribed in a spatiotemporal manner over the course of spermatocyte differentiation, which spans ~90 hours. The introns of these genes contain megabases of simple satellite DNA repeats that comprise over 99% of the gene loci, and these satellite-DNA containing introns are transcribed. We identify two RNA-binding proteins that specifically localize to kl-3 and kl-5 transcripts and are needed for the successful transcription or processing of these genes. We propose that genes with intron gigantism require a unique gene expression program, which may serve as a platform to regulate gene expression during cellular differentiation.

PubMed ID
PubMed Central ID
PMC6508621 (PMC) (EuropePMC)
Related Publication(s)
Note

Taming the giant within.
Bateman and Anderson, 2019, PLoS Genet. 15(5): e1008098 [FBrf0242842]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    PLoS Genet.
    Title
    PLoS Genetics
    Publication Year
    2005-
    ISBN/ISSN
    1553-7404 1553-7390
    Data From Reference
    Aberrations (2)
    Alleles (121)
    Genes (73)
    Insertions (43)
    Experimental Tools (1)
    Transgenic Constructs (62)