Open Close
Reference
Citation
Kockel, L., Griffin, C., Ahmed, Y., Fidelak, L., Rajan, A., Gould, E.P., Haigney, M., Ralston, B., Tercek, R.J., Galligani, L., Rao, S., Huq, L., Bhargava, H.K., Dooner, A.C., Lemmerman, E.G., Malusa, R.F., Nguyen, T.H., Chung, J.S., Gregory, S.M., Kuwana, K.M., Regenold, J.T., Wei, A., Ashton, J., Dickinson, P., Martel, K., Cai, C., Chen, C., Price, S., Qiao, J., Shepley, D., Zhang, J., Chalasani, M., Nguyen, K., Aalto, A., Kim, B., Tazawa-Goodchild, E., Sherwood, A., Rahman, A., Wu, S.Y.C., Lotzkar, J., Michaels, S., Aristotle, H., Clark, A., Gasper, G., Xiang, E., Schlör, F.L., Lu, M., Haering, K., Friberg, J., Kuwana, A., Lee, J., Liu, A., Norton, E., Hamad, L., Lee, C., Okeremi, D., diTullio, H., Dumoulin, K., Chi, S.Y.G., Derossi, G.S., Horowitch, R.E., Issa, E.C., Le, D.T., Morales, B.C., Noori, A., Shao, J., Cho, S., Hoang, M.N., Johnson, I.M., Lee, K.C., Lee, M., Madamidola, E.A., Schmitt, K.E., Byan, G., Park, T., Chen, J., Monovoukas, A., Kang, M.J., McGowan, T., Walewski, J.J., Simon, B., Zu, S.J., Miller, G.P., Fitzpatrick, K.B., Lantz, N., Fox, E., Collette, J., Kurtz, R., Duncan, C., Palmer, R., Rotondo, C., Janicki, E., Chisholm, T., Rankin, A., Park, S., Kim, S.K. (2019). An Interscholastic Network to Generate LexA Enhancer Trap Lines in Drosophila.  G3 (Bethesda) (): .
FlyBase ID
FBrf0242321
Publication Type
Research paper
Abstract

Binary expression systems like the LexA-LexAop system provide a powerful experimental tool kit to study gene and tissue function in developmental biology, neurobiology and physiology. However, the number of well-defined LexA enhancer trap insertions remains limited. In this study, we present the molecular characterization and initial tissue expression analysis of nearly 100 novel StanEx LexA enhancer traps, derived from the StanEx1 index line. This includes 76 insertions into novel, distinct gene loci not previously associated with enhancer traps or targeted LexA constructs. Additionally, our studies revealed evidence for selective transposase-dependent replacement of a previously-undetected KP element on chromosome III within the StanEx1 genetic background during hybrid dysgenesis, suggesting a molecular basis for the over-representation of LexA insertions at the NK7.1 locus in our screen. Production and characterization of novel fly lines were performed by students and teachers in experiment-based genetics classes within a geographically diverse network of public and independent high schools. Thus, unique partnerships between secondary schools and university-based programs have produced and characterized novel genetic and molecular resources in Drosophila for open-source distribution, and provide paradigms for development of science education through experience-based pedagogy.

PubMed ID
PubMed Central ID
PMC6643891 (PMC) (EuropePMC)
Related Publication(s)
Personal communication to FlyBase

StanEx Stocks.
Kockel, 2019.4.9, StanEx Stocks. [FBrf0242323]

Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    G3 (Bethesda)
    Title
    G3 : genes - genomes - genetics
    ISBN/ISSN
    2160-1836
    Data From Reference
    Alleles (184)
    Genes (100)
    Insertions (107)
    Transgenic Constructs (2)