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Citation
Robles-Murguia, M., Hunt, L.C., Finkelstein, D., Fan, Y., Demontis, F. (2019). Tissue-specific alteration of gene expression and function by RU486 and the GeneSwitch system.  NPJ Aging Mech. Dis. 5(): 6.
FlyBase ID
FBrf0242458
Publication Type
Research paper
Abstract

The GeneSwitch (GS) is a modified Gal4/UAS system, whereby transgene expression is induced in Drosophila by adding the drug RU486 to food. The GS system is routinely used in Drosophila aging and behavioral studies to avoid confounding effects related to genetic background mutations. Here, we report transcriptional and functional defects that are induced by RU486 in a stock- and tissue-dependent manner, such as defects in flight and mitochondrial gene expression. In addition to including proper controls, our findings suggest that context-specific side effects induced by RU486 should be considered in the experimental design and when interpreting the observed phenotypes.

PubMed ID
PubMed Central ID
PMC6529481 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    NPJ Aging Mech. Dis.
    Title
    NPJ aging and mechanisms of disease
    ISBN/ISSN
    2056-3973
    Data From Reference
    Alleles (7)
    Genes (3)
    Natural transposons (1)
    Insertions (2)
    Experimental Tools (1)
    Transgenic Constructs (5)