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Citation
Lapart, J.A., Gottardo, M., Cortier, E., Duteyrat, J.L., Augière, C., Mangé, A., Jerber, J., Solassol, J., Gopalakrishnan, J., Thomas, J., Durand, B. (2019). Dzip1 and Fam92 form a ciliary transition zone complex with cell type specific roles in Drosophila.  eLife 8(): e49307.
FlyBase ID
FBrf0244294
Publication Type
Research paper
Abstract

Cilia and flagella are conserved eukaryotic organelles essential for cellular signaling and motility. Cilia dysfunctions cause life-threatening ciliopathies, many of which are due to defects in the transition zone (TZ), a complex structure of the ciliary base. Therefore, understanding TZ assembly, which relies on ordered interactions of multiprotein modules, is of critical importance. Here, we show that Drosophila Dzip1 and Fam92 form a functional module which constrains the conserved core TZ protein, Cep290, to the ciliary base. We identify cell type specific roles of this functional module in two different tissues. While it is required for TZ assembly in all Drosophila ciliated cells, it also regulates basal-body growth and docking to the plasma membrane during spermatogenesis. We therefore demonstrate a novel regulatory role for Dzip1 and Fam92 in mediating membrane/basal-body interactions and show that these interactions exhibit cell type specific functions in basal-body maturation and TZ organization.

PubMed ID
PubMed Central ID
PMC6904220 (PMC) (EuropePMC)
Related Publication(s)
Personal communication to FlyBase

Location data for DZIP1 and Fam92 deletions.
Thomas, 2020.3.16, Location data for DZIP1 and Fam92 deletions. [FBrf0245158]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    eLife
    Title
    eLife
    ISBN/ISSN
    2050-084X
    Data From Reference
    Aberrations (2)
    Alleles (11)
    Genes (8)
    Physical Interactions (3)
    Natural transposons (1)
    Insertions (4)
    Experimental Tools (11)
    Transgenic Constructs (7)