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Citation
Li, J., Han, S., Li, H., Udeshi, N.D., Svinkina, T., Mani, D.R., Xu, C., Guajardo, R., Xie, Q., Li, T., Luginbuhl, D.J., Wu, B., McLaughlin, C.N., Xie, A., Kaewsapsak, P., Quake, S.R., Carr, S.A., Ting, A.Y., Luo, L. (2020). Cell-Surface Proteomic Profiling in the Fly Brain Uncovers Wiring Regulators.  Cell 180(2): 373--386.e15.
FlyBase ID
FBrf0244620
Publication Type
Research paper
Abstract

Molecular interactions at the cellular interface mediate organized assembly of single cells into tissues and, thus, govern the development and physiology of multicellular organisms. Here, we developed a cell-type-specific, spatiotemporally resolved approach to profile cell-surface proteomes in intact tissues. Quantitative profiling of cell-surface proteomes of Drosophila olfactory projection neurons (PNs) in pupae and adults revealed global downregulation of wiring molecules and upregulation of synaptic molecules in the transition from developing to mature PNs. A proteome-instructed in vivo screen identified 20 cell-surface molecules regulating neural circuit assembly, many of which belong to evolutionarily conserved protein families not previously linked to neural development. Genetic analysis further revealed that the lipoprotein receptor LRP1 cell-autonomously controls PN dendrite targeting, contributing to the formation of a precise olfactory map. These findings highlight the power of temporally resolved in situ cell-surface proteomic profiling in discovering regulators of brain wiring.

PubMed ID
PubMed Central ID
PMC7072036 (PMC) (EuropePMC)
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Cell
    Title
    Cell
    Publication Year
    1974-
    ISBN/ISSN
    0092-8674
    Data From Reference
    Alleles (56)
    Genes (26)
    Natural transposons (1)
    Insertions (5)
    Experimental Tools (5)
    Transgenic Constructs (12)