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Weiner, A.T., Seebold, D.Y., Torres-Gutierrez, P., Folker, C., Swope, R.D., Kothe, G.O., Stoltz, J.G., Zalenski, M.K., Kozlowski, C., Barbera, D.J., Patel, M.A., Thyagarajan, P., Shorey, M., Nye, D.M.R., Keegan, M., Behari, K., Song, S., Axelrod, J.D., Rolls, M.M. (2020). Endosomal Wnt signaling proteins control microtubule nucleation in dendrites.  PLoS Biol. 18(3): e3000647.
FlyBase ID
FBrf0245140
Publication Type
Research paper
Abstract

Dendrite microtubules are polarized with minus-end-out orientation in Drosophila neurons. Nucleation sites concentrate at dendrite branch points, but how they localize is not known. Using Drosophila, we found that canonical Wnt signaling proteins regulate localization of the core nucleation protein γTubulin (γTub). Reduction of frizzleds (fz), arrow (low-density lipoprotein receptor-related protein LRP 5/6), dishevelled (dsh), casein kinase Iγ, G proteins, and Axin reduced γTub-green fluorescent protein (GFP) at branch points, and two functional readouts of dendritic nucleation confirmed a role for Wnt signaling proteins. Both dsh and Axin localized to branch points, with dsh upstream of Axin. Moreover, tethering Axin to mitochondria was sufficient to recruit ectopic γTub-GFP and increase microtubule dynamics in dendrites. At dendrite branch points, Axin and dsh colocalized with early endosomal marker Rab5, and new microtubule growth initiated at puncta marked with fz, dsh, Axin, and Rab5. We propose that in dendrites, canonical Wnt signaling proteins are housed on early endosomes and recruit nucleation sites to branch points.

PubMed ID
PubMed Central ID
PMC7067398 (PMC) (EuropePMC)
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    PLoS Biol.
    Title
    PLoS Biology
    Publication Year
    2003-
    ISBN/ISSN
    1545-7885 1544-9173
    Data From Reference
    Aberrations (1)
    Alleles (69)
    Genes (40)
    Natural transposons (1)
    Insertions (1)
    Experimental Tools (9)
    Transgenic Constructs (14)