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Citation
Petzoldt, A.G., Götz, T.W.B., Driller, J.H., Lützkendorf, J., Reddy-Alla, S., Matkovic-Rachid, T., Liu, S., Knoche, E., Mertel, S., Ugorets, V., Lehmann, M., Ramesh, N., Beuschel, C.B., Kuropka, B., Freund, C., Stelzl, U., Loll, B., Liu, F., Wahl, M.C., Sigrist, S.J. (2020). RIM-binding protein couples synaptic vesicle recruitment to release sites.  J. Cell Biol. 219(7): e201902059.
FlyBase ID
FBrf0245633
Publication Type
Research paper
Abstract

At presynaptic active zones, arrays of large conserved scaffold proteins mediate fast and temporally precise release of synaptic vesicles (SVs). SV release sites could be identified by clusters of Munc13, which allow SVs to dock in defined nanoscale relation to Ca2+ channels. We here show in Drosophila that RIM-binding protein (RIM-BP) connects release sites physically and functionally to the ELKS family Bruchpilot (BRP)-based scaffold engaged in SV recruitment. The RIM-BP N-terminal domain, while dispensable for SV release site organization, was crucial for proper nanoscale patterning of the BRP scaffold and needed for SV recruitment of SVs under strong stimulation. Structural analysis further showed that the RIM-BP fibronectin domains form a "hinge" in the protein center, while the C-terminal SH3 domain tandem binds RIM, Munc13, and Ca2+ channels release machinery collectively. RIM-BPs' conserved domain architecture seemingly provides a relay to guide SVs from membrane far scaffolds into membrane close release sites.

PubMed ID
PubMed Central ID
PMC7337501 (PMC) (EuropePMC)
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    J. Cell Biol.
    Title
    Journal of Cell Biology
    Publication Year
    1966-
    ISBN/ISSN
    0021-9525
    Data From Reference
    Aberrations (3)
    Alleles (23)
    Genes (8)
    Physical Interactions (15)
    Natural transposons (1)
    Insertions (20)
    Experimental Tools (3)
    Transgenic Constructs (15)