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Zhang, B., Binks, T., Burke, R. (2020). The E3 ubiquitin ligase Slimb/β-TrCP is required for normal copper homeostasis in Drosophila.  Biochim Biophys Acta Mol Cell Res 1867(10): 118768.
FlyBase ID
FBrf0246444
Publication Type
Research paper
Abstract

The Drosophila Slimb (Slmb) gene encodes a Skp1-Cul1-F-box (SCP) E3 ubiquitin ligase orthologous to the human β-TrCP/BTRC protein. Slmb and/or BTRC play regulatory roles in numerous biological processes by ubiquitinating several substrate proteins which are then targeted for proteasomal degradation. Here, we demonstrate an additional role for Slmb in maintaining cellular copper homeostasis. In the thorax, midgut and eye, Slmb knockdown causes copper deficiency phenotypes which can be rescued by increasing cellular copper levels via decreased efflux or increased uptake. Furthermore, Slmb knockdown results in decreased levels of the copper transporters Ctr1A and ATP7, indicating Slmb is required to regulate copper homeostasis. We also present evidence that the transcription factor Cap-n-Collar (Nrf2 in mammals), a known substrate of Slmb/BTRC, mediates Slmb's regulatory effect on Ctr1A in a post-transcriptional manner.

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    Language of Publication
    English
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    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Biochim Biophys Acta Mol Cell Res
    Title
    Biochimica et biophysica acta. Molecular cell research.
    ISBN/ISSN
    1879-2596 0167-4889
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