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Citation
Linnemannstöns, K., Witte, L., Karuna M, P., Kittel, J.C., Danieli, A., Müller, D., Nitsch, L., Honemann-Capito, M., Grawe, F., Wodarz, A., Gross, J.C. (2020). Ykt6-dependent endosomal recycling is required for Wnt secretion in the Drosophila wing epithelium.  Development 147(15): dev185421.
FlyBase ID
FBrf0246471
Publication Type
Research paper
Abstract

Morphogens are important signalling molecules for tissue development and their secretion requires tight regulation. In the wing imaginal disc of flies, the morphogen Wnt/Wingless is apically presented by the secreting cell and re-internalized before final long-range secretion. Why Wnt molecules undergo these trafficking steps and the nature of the regulatory control within the endosomal compartment remain unclear. Here, we have investigated how Wnts are sorted at the level of endosomes by the versatile v-SNARE Ykt6. Using in vivo genetics, proximity-dependent proteomics and in vitro biochemical analyses, we show that most Ykt6 is present in the cytosol, but can be recruited to de-acidified compartments and recycle Wnts to the plasma membrane via Rab4-positive recycling endosomes. Thus, we propose a molecular mechanism by which producing cells integrate and leverage endocytosis and recycling via Ykt6 to coordinate extracellular Wnt levels.

PubMed ID
PubMed Central ID
PMC7438013 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Development
    Title
    Development
    Publication Year
    1987-
    ISBN/ISSN
    0950-1991
    Data From Reference