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Cho, B., Yoon, S.H., Lee, D., Koranteng, F., Tattikota, S.G., Cha, N., Shin, M., Do, H., Hu, Y., Oh, S.Y., Lee, D., Vipin Menon, A., Moon, S.J., Perrimon, N., Nam, J.W., Shim, J. (2020). Single-cell transcriptome maps of myeloid blood cell lineages in Drosophila.  Nat. Commun. 11(1): 4483.
FlyBase ID
FBrf0246655
Publication Type
Research paper
Abstract

The Drosophila lymph gland, the larval hematopoietic organ comprised of prohemocytes and mature hemocytes, has been a valuable model for understanding mechanisms underlying hematopoiesis and immunity. Three types of mature hemocytes have been characterized in the lymph gland: plasmatocytes, lamellocytes, and crystal cells, which are analogous to vertebrate myeloid cells, yet molecular underpinnings of the lymph gland hemocytes have been less investigated. Here, we use single-cell RNA sequencing to comprehensively analyze heterogeneity of developing hemocytes in the lymph gland, and discover previously undescribed hemocyte types including adipohemocytes, stem-like prohemocytes, and intermediate prohemocytes. Additionally, we identify the developmental trajectory of hemocytes during normal development as well as the emergence of the lamellocyte lineage following active cellular immunity caused by wasp infestation. Finally, we establish similarities and differences between embryonically derived- and larval lymph gland hemocytes. Altogether, our study provides detailed insights into the hemocyte development and cellular immune responses at single-cell resolution.

PubMed ID
PubMed Central ID
PMC7479620 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Nat. Commun.
    Title
    Nature communications
    ISBN/ISSN
    2041-1723
    Data From Reference
    Alleles (109)
    Genes (278)
    Natural transposons (1)
    Insertions (71)
    Experimental Tools (4)
    Transgenic Constructs (71)