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Citation
Sawyer, J.K., Kabiri, Z., Montague, R.A., Allen, S.R., Stewart, R., Paramore, S.V., Cohen, E., Zaribafzadeh, H., Counter, C.M., Fox, D.T. (2020). Exploiting codon usage identifies intensity-specific modifiers of Ras/MAPK signaling in vivo.  PLoS Genet. 16(12): e1009228.
FlyBase ID
FBrf0247629
Publication Type
Research paper
Abstract

Signal transduction pathways are intricately fine-tuned to accomplish diverse biological processes. An example is the conserved Ras/mitogen-activated-protein-kinase (MAPK) pathway, which exhibits context-dependent signaling output dynamics and regulation. Here, by altering codon usage as a novel platform to control signaling output, we screened the Drosophila genome for modifiers specific to either weak or strong Ras-driven eye phenotypes. Our screen enriched for regions of the genome not previously connected with Ras phenotypic modification. We mapped the underlying gene from one modifier to the ribosomal gene RpS21. In multiple contexts, we show that RpS21 preferentially influences weak Ras/MAPK signaling outputs. These data show that codon usage manipulation can identify new, output-specific signaling regulators, and identify RpS21 as an in vivo Ras/MAPK phenotypic regulator.

PubMed ID
PubMed Central ID
PMC7752094 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    PLoS Genet.
    Title
    PLoS Genetics
    Publication Year
    2005-
    ISBN/ISSN
    1553-7404 1553-7390
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    Experimental Tools (3)
    Transgenic Constructs (7)