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Citation
McLaughlin, C.N., Brbić, M., Xie, Q., Li, T., Horns, F., Kolluru, S.S., Kebschull, J.M., Vacek, D., Xie, A., Li, J., Jones, R.C., Leskovec, J., Quake, S.R., Luo, L., Li, H. (2021). Single-cell transcriptomes of developing and adult olfactory receptor neurons in Drosophila.  eLife 10(): e63856.
FlyBase ID
FBrf0248018
Publication Type
Research paper
Abstract

Recognition of environmental cues is essential for the survival of all organisms. Transcriptional changes occur to enable the generation and function of the neural circuits underlying sensory perception. To gain insight into these changes, we generated single-cell transcriptomes of Drosophila olfactory- (ORNs), thermo-, and hygro-sensory neurons at an early developmental and adult stage using single-cell and single-nucleus RNA sequencing. We discovered that ORNs maintain expression of the same olfactory receptors across development. Using receptor expression and computational approaches, we matched transcriptomic clusters corresponding to anatomically and physiologically defined neuron types across multiple developmental stages. We found that cell-type-specific transcriptomes partly reflected axon trajectory choices in development and sensory modality in adults. We uncovered stage-specific genes that could regulate the wiring and sensory responses of distinct ORN types. Collectively, our data reveal transcriptomic features of sensory neuron biology and provide a resource for future studies of their development and physiology.

PubMed ID
PubMed Central ID
PMC7870146 (PMC) (EuropePMC)
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    eLife
    Title
    eLife
    ISBN/ISSN
    2050-084X
    Data From Reference