FB2025_01 , released February 20, 2025
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Marelja, Z., Jouandin, P. (2022.4.8). Location data for Ctns[KO]. 
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FBrf0253308
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Personal communication to FlyBase
Abstract
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The CtnsKO frameshift mutation is located within exon 4 of the Ctns coding sequence (counting from the ATG at bp position 252). The CRISPR/Cas9-induced frameshift mutation creates a 5bp  indel mutation (7 nucleotides deleted, 2 extra nucelotides inserted).
The translated sequence of the wildtype Ctns protein GLLDSDVEVALGTDSEDHLLLDPATFVYPAGSTRNQSVVITGLKAGNVKVVADSDDANKEI
is changed into
GLLDSDVEVALGTDSEDHLLLDPATFVYPAGSTPVGGDNWPQSRQRQSGRR*R* 
in the CtnsKO frameshift mutant generating a nonsense amino acid sequence after PAGST followed by 18 novel amino acids and two premature STOP (*) codons prior to the first transmembrane domain. See the associated files for the wild type and mutant sequences.
DOI
Related Publication(s)
Research paper

Lysosomal cystine mobilization shapes the response of TORC1 and tissue growth to fasting.
Jouandin et al., 2022, Science 375(6582): eabc4203 [FBrf0252719]

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File date: 2022.4.8 ; File size: 22312 ; File format: docx ; File name: Marelja.22.4.8_Ctns_mutant_sequence.docx
File date: 2022.4.8 ; File size: 183894 ; File format: jpeg ; File name: Marelja.22.4.8_Ctns_mutation.jpeg
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    English
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