A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Sequence Feature: RNAi_reagent

General Information
Symbol dsRNA-GD558 Species D. melanogaster
Feature type RNAi_reagent FlyBase ID FBsf0000071369
Collection VDRC-1   Associated gene(s) Acph-1
Genomic Location
Chromosome (arm) 3R Sequence location 3R:25,818,011..25,818,298 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBtr0085545 FBtr0085610 FBtr0085609 FBtr0085608 FBtr0334707 FBsf0000035821 FBsf0000013598 FBsf0000027504 FBsf0000015823 FBsf0000194562 FBsf0000071369 FBsf0000088148 FBsf0000080732 FBsf0000415490 FBsf0000400720 FBsf0000415214 FBsf0000413238 FBsf0000401911 FBsf0000393305 FBsf0000395415
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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hide Sequence Data
Length 288
Comments
One copy of repeat shown
(Dietzl et al., 2007)
Sequence 
GGCAACCCATACCCATTCACACCTCGCCCGAGAGGGAGGATCCCATACTG
GCCGCTAAGGCACCTTGTCCTGCCTATGACTATGAGCTGGCCAGCCTGGA
ATCATCGCCAGAATTCAAGGCCTTGACCGAAAAACACCGAAATCTGTTTG
CTTATTTGAGCGAAAAAGGCGGACGCCCCGTGAAGACCTTCATTGATGCC
CAGTATTTGAATAACACCTTGTTCATTGAGAATCTGTACAACATGACACT
GCCAAAATGGACTAAAATGGTTTATGGGAGAGAGGAGC
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Gene(s)
(targeted or local)
Allele(s)
Transcripts(s)
Polypeptide(s)
Construct
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PCR template
genomic DNA
(Dietzl et al., 2007)
Primer 1
GGCAACCCATACCCATTCACACC
(Dietzl et al., 2007)
Primer 2
GCTCCTCTCTCCCATAAACCATTTTAGTCC
(Dietzl et al., 2007)
Comments
hide Collection Information
hide Collection: VDRC-1
Symbol VDRC-1
Source and Progenitors
Species of derivation D. melanogaster  
Strain of derivation
Vector or progenitor construct
Description
Comprehensive collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene.
(Dietzl et al., 2007)
Additional data
A collection of over 13,200 RNAi transgenes (over 22,200 lines), each designed to target a single protein-coding gene; each construct contains short gene fragments cloned as inverted repeats that are expressed using the binary GAL4/UAS system. It is estimated that 88% of predicted protein-coding genes are targeted.
(Dietzl et al., 2007)
Cell lines
Observed in
Not observed in
hide Comments
hide Stocks Listed in FlyBase ( 1 )
VDRC
v3579
w1118; P{GD558}v3579/TM3
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hide Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym
558
(Dietzl et al., 2007)
dsRNA-GD558
 
Secondary FlyBase IDs
  • FBri0067842
hide References ( 1 )
Supplementary material
Dietzl et al., 2007, Nature 448(7150):
Supplementary Table 1. [FBrf0202579]