A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Sequence Feature: RNAi_reagent

General Information
Symbol dsRNA-GD3478 Species D. melanogaster
Feature type RNAi_reagent FlyBase ID FBsf0000073968
Collection VDRC-1   Associated gene(s)
Genomic Location
Chromosome (arm) 2L Sequence location 2L:21,167,786..21,168,153 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBtr0081472 FBtr0306976 FBtr0306974 FBtr0306975 FBtr0081473 FBtr0300425 FBsf0000003065 FBsf0000073968 FBsf0000151954
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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hide Sequence Data
Length 368
Comments
One copy of repeat shown
(Dietzl et al., 2007)
Sequence 
CGGAAGAGCCAACCCCTTTGACCACATCGTGTTGCCGGCGTCCCAATTGC
GGCCTAGGGCTCCAGGATGAGCGAAATATCTGTTCCTGCAAGCACGGCTA
CATCATACCAGTGCTTATACTCTTCGTTCTGGTGATGATTGTGCTTCTGT
TGCCTTGGGAGACCTACCATCGAAATGAAAACGGAAACAGCCCCGTTCCG
GTGGAAAAGCCGTTACCATGTCAACTCGAACTGGTGGAGAGCTTGCCCTT
GGGGTTGAACTACAGCCAATGGCAAAGCCACCCCCAACTGAGTAGCACCT
TTGATGCCTGGCAGCTACTACTAGGCAGGGCCAAGGCATCGTTGGACATC
GTTTCTCCGCACTGGACA
hide Associated Information
Gene(s)
(targeted or local)
Allele(s)
Transcripts(s)
Polypeptide(s)
Construct
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hide Progenitors
PCR template
genomic DNA
(Dietzl et al., 2007)
Primer 1
CGGAAGAGCCAACCCCTTTGA
(Dietzl et al., 2007)
Primer 2
TGTCCAGTGCGGAGAAACGATG
(Dietzl et al., 2007)
Comments
hide Collection Information
hide Collection: VDRC-1
Symbol VDRC-1
Source and Progenitors
Species of derivation D. melanogaster  
Strain of derivation
Vector or progenitor construct
Description
Comprehensive collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene.
(Dietzl et al., 2007)
Additional data
A collection of over 13,200 RNAi transgenes (over 22,200 lines), each designed to target a single protein-coding gene; each construct contains short gene fragments cloned as inverted repeats that are expressed using the binary GAL4/UAS system. It is estimated that 88% of predicted protein-coding genes are targeted.
(Dietzl et al., 2007)
Cell lines
Observed in
Not observed in
hide Comments
hide Stocks Listed in FlyBase ( 2 )
VDRC
v37422
w1118; P{GD3478}v37422
v37423
w1118; P{GD3478}v37423
hide External Crossreferences & Linkouts
hide Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym
3478
(Dietzl et al., 2007)
dsRNA-GD3478
 
Secondary FlyBase IDs
  • FBri0070589
hide References ( 1 )
Supplementary material
Dietzl et al., 2007, Nature 448(7150):
Supplementary Table 1. [FBrf0202579]