A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Sequence Feature: RNAi_reagent

General Information
Symbol dsRNA-GD8256 Species D. melanogaster
Feature type RNAi_reagent FlyBase ID FBsf0000077460
Collection VDRC-1   Associated gene(s) CG14200
Genomic Location
Chromosome (arm) X Sequence location X:19,409,153..19,409,505 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBtr0074721 FBtr0307196 FBtr0307195 FBsf0000030622 FBsf0000018957 FBsf0000077460 FBsf0000092815 FBsf0000408518 FBsf0000397693
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
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This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Length 353
Comments
One copy of repeat shown
(Dietzl et al., 2007)
Sequence 
CGGGACGGGGAAGAGAAGGAGACTGGGGTCGGCTCCGAAGGTGAACAGCC
CGCAATGGCTACGGATGAAGAAGAAGTATCTGACGACAGGAGGTTCCAGA
TGGCAATGGACATGGACATCGCACTGGATGCCGTGGAGCTGGTGATGCCG
GTTTCATTGCTGCAAACCTCGCGCCGCCCTGCCAGCTACCCGCAAGTCAC
TCCGATGGCGACCACCCAGTCGGCCATTGGCCTAAATCGACCCACCAGGC
ATTTAAGCTGTGCCCGAGCCCTGAGCGATGCGGACATGGCCAGGGCCCAC
CAGCAGCTGGTGGATCTGCGCGAGCAGCAGCGCCTCTGCTTCACCCCGCC
ATC
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Gene(s)
(targeted or local)
Allele(s)
Transcripts(s)
Polypeptide(s)
Construct
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PCR template
genomic DNA
(Dietzl et al., 2007)
Primer 1
CGGGACGGGGAAGAGAAGGA
(Dietzl et al., 2007)
Primer 2
GATGGCGGGGTGAAGCAGAG
(Dietzl et al., 2007)
Comments
hide Collection Information
hide Collection: VDRC-1
Symbol VDRC-1
Source and Progenitors
Species of derivation D. melanogaster  
Strain of derivation
Vector or progenitor construct
Description
Comprehensive collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene.
(Dietzl et al., 2007)
Additional data
A collection of over 13,200 RNAi transgenes (over 22,200 lines), each designed to target a single protein-coding gene; each construct contains short gene fragments cloned as inverted repeats that are expressed using the binary GAL4/UAS system. It is estimated that 88% of predicted protein-coding genes are targeted.
(Dietzl et al., 2007)
Cell lines
Observed in
Not observed in
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hide Stocks Listed in FlyBase ( 0 )
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hide Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym
8256
(Dietzl et al., 2007)
dsRNA-GD8256
 
Secondary FlyBase IDs
  • FBri0074883
hide References ( 1 )
Supplementary material
Dietzl et al., 2007, Nature 448(7150):
Supplementary Table 1. [FBrf0202579]