One copy of repeat shown
ACGAGATACAGAAAGGCGGAAAGGATGAGTCCTCTGCACCACCGCTTCCCAAAACGGGGCCACCAGCCACAGTTCCCAGG AAAGTATATCGCCAGGATTTGGTCATCAACGTAGAGCCAGCACCCAGTTTAACCAGGGACTATCAGAGATCTCTGAGTGG TGGTACTCCCCGGAAGCCTAGCGATTGGCGCAAGGATGAAAAGTCGGAGAAATCAGTGCGCGACAAGATTGCCATGTTCT CGTCAAACAACGAACTGGATGCCATACCGCCAGCTCCGGCAACAGCCCCCATTTCAAGCTCCTTCACCCGGAAGCCCTTG AATAGGAGCAGTGAAAATCTTCTGGACAGTTGCTCATCATCCTCGGCTCCTTCGCTG
A collection of over 13,200 RNAi transgenes (over 22,200 lines), each designed to target a single protein-coding gene; each construct contains short gene fragments cloned as inverted repeats that are expressed using the binary GAL4/UAS system. It is estimated that 88% of predicted protein-coding genes are targeted.
A specificity score (Q) was calculated for each construct, such that if Q is < 0.5, off-target effects are probable and if Q > 0.8, off-target effects are improbable.