A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Sequence Feature: RNAi_reagent

General Information
Symbol dsRNA-GD10226 Species D. melanogaster
Feature type RNAi_reagent FlyBase ID FBsf0000084463
Collection VDRC-1   Associated gene(s)
Genomic Location
Chromosome (arm) 3R Sequence location 3R:14,080,100..14,080,472 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBtr0083605 FBtr0310380 FBtr0310379 FBtr0310381 FBtr0310382 FBtr0310383 FBtr0310384 FBtr0299938 FBtr0299939 FBsf0000013992 FBsf0000020398 FBsf0000032543 FBsf0000015691 FBsf0000015856 FBsf0000035185 FBsf0000022316 FBsf0000076210 FBsf0000084463 FBsf0000082047 FBsf0000409424 FBsf0000393677 FBsf0000395290 FBsf0000429424 FBsf0000431700
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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hide Sequence Data
Length 373
Comments
One copy of repeat shown
(Dietzl et al., 2007)
Sequence 
GCCACGAACCCGCAGAAGTGCGTCGTCACTACCACGATTGCTACTTTGGC
GGGATATTCGAGCGGCTCCTTAATCTGAAGCATGCGAGGGACAGCTATGT
GCCCGAGCGAATGCCGTATGTCTTCAAAATGCGCAGCTTGGATCCACCAC
GTCATGACGACATCGCCTCCATGCAGTTCAGACTAAGCGCAGGTTACCGC
TGTGCAAGAGGAGACTTCGATACTCCGTACGACACCTCAGCAGAGAGTCT
GCTATCTATTATGGTGGATCATAGGGGCAGGGATGACGACAACGAGGCGT
CTGAGAGTGAGTTTGAGCGCGAAGTGACAGAGGAATTGCAACTCGGACTA
GTTCGGGCATACAACAATCGCCT
hide Associated Information
Gene(s)
(targeted or local)
Allele(s)
Transcripts(s)
Polypeptide(s)
Construct
hide Experimental Data
hide Progenitors
PCR template
genomic DNA
(Dietzl et al., 2007)
Primer 1
GCCACGAACCCGCAGAAGTG
(Dietzl et al., 2007)
Primer 2
AGGCGATTGTTGTATGCCCGAAC
(Dietzl et al., 2007)
Comments
hide Collection Information
hide Collection: VDRC-1
Symbol VDRC-1
Source and Progenitors
Species of derivation D. melanogaster  
Strain of derivation
Vector or progenitor construct
Description
Comprehensive collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene.
(Dietzl et al., 2007)
Additional data
A collection of over 13,200 RNAi transgenes (over 22,200 lines), each designed to target a single protein-coding gene; each construct contains short gene fragments cloned as inverted repeats that are expressed using the binary GAL4/UAS system. It is estimated that 88% of predicted protein-coding genes are targeted.
(Dietzl et al., 2007)
Cell lines
Observed in
Not observed in
hide Comments
hide Stocks Listed in FlyBase ( 1 )
VDRC
v21985
w1118; P{GD10226}v21985
hide External Crossreferences & Linkouts
hide Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym
10226
(Dietzl et al., 2007)
dsRNA-GD10226
 
Secondary FlyBase IDs
  • FBri0076651
hide References ( 1 )
Supplementary material
Dietzl et al., 2007, Nature 448(7150):
Supplementary Table 1. [FBrf0202579]