A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Sequence Feature: RNAi_reagent

General Information
Symbol dsRNA-GD13442 Species D. melanogaster
Feature type RNAi_reagent FlyBase ID FBsf0000085064
Collection VDRC-1   Associated gene(s) Mical
Genomic Location
Chromosome (arm) 3R Sequence location 3R:5,828,648..5,829,138 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBtr0300257 FBtr0306611 FBtr0082215 FBtr0082213 FBtr0082212 FBtr0082211 FBtr0082210 FBtr0306609 FBtr0082207 FBtr0306608 FBtr0306610 FBtr0082209 FBtr0082208 FBsf0000015267 FBsf0000013845 FBsf0000091167 FBsf0000406153 FBsf0000394873 FBsf0000393540
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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hide Sequence Data
Length 310
Comments
One copy of repeat shown
(Dietzl et al., 2007)
Sequence 
CCACCCATACCGCCTCTTCCACTGAATTATCAGAGATCCGATGATGAGAG
CTACGCTAACGAGACACGAGAGCATAAGAAGCAACGTGCCATATCGAAGG
CTTCACGACAAGCTGAGCTCAAGCGATTGCGAATCGCTCAAGAGATTCAG
CGGGAACAGGAGGAGATCGAGGTGCAACTGAAGGATCTGGAGGCACGCGG
CGTGCTTATTGAGAAGGCCTTGCGAGGCGAGGCGCAGAATATTGAAAACC
TGGATGCGACAAAGGACAACGACGAGAAGCTACTTAAGGAACTTTTGGAG
ATTTGGCGCA
hide Associated Information
Gene(s)
(targeted or local)
Allele(s)
Transcripts(s)
Polypeptide(s)
Construct
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PCR template
cDNA
(Dietzl et al., 2007)
Primer 1
CCACCCATACCGCCTCTTCCA
(Dietzl et al., 2007)
Primer 2
TGCGCCAAATCTCCAAAAGTTCC
(Dietzl et al., 2007)
Comments
hide Collection Information
hide Collection: VDRC-1
Symbol VDRC-1
Source and Progenitors
Species of derivation D. melanogaster  
Strain of derivation
Vector or progenitor construct
Description
Comprehensive collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene.
(Dietzl et al., 2007)
Additional data
A collection of over 13,200 RNAi transgenes (over 22,200 lines), each designed to target a single protein-coding gene; each construct contains short gene fragments cloned as inverted repeats that are expressed using the binary GAL4/UAS system. It is estimated that 88% of predicted protein-coding genes are targeted.
(Dietzl et al., 2007)
Cell lines
Observed in
Not observed in
hide Comments
hide Stocks Listed in FlyBase ( 2 )
VDRC
v46096
w1118; P{GD13442}v46096
v46097
w1118; P{GD13442}v46097
hide External Crossreferences & Linkouts
hide Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym
13442
(Dietzl et al., 2007)
dsRNA-GD13442
 
Secondary FlyBase IDs
  • FBri0079323
hide References ( 1 )
Supplementary material
Dietzl et al., 2007, Nature 448(7150):
Supplementary Table 1. [FBrf0202579]