A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Sequence Feature: RNAi_reagent

General Information
Symbol dsRNA-JF02010 Species D. melanogaster
Feature type RNAi_reagent FlyBase ID FBsf0000086191
Collection TRiP-1   Associated gene(s) Trn-SR
Genomic Location
Chromosome (arm) 2L Sequence location 2L:2,762,390..2,762,913 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBtr0335126 FBtr0077715 FBtr0077736 FBtr0335129 FBsf0000035603 FBsf0000000532 FBsf0000034097 FBsf0000026057 FBsf0000000550 FBsf0000078668 FBsf0000194421 FBsf0000078919 FBsf0000086191 FBsf0000400468 FBsf0000400048 FBsf0000384650 FBsf0000384666
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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hide Sequence Data
Length 524
Comments
One copy of repeat shown.
(Perkins et al., 1.15.2009)
Sequence 
GATGGTGAGAAAGCAAGCAATGATGCTGGTGGAACCGCTGATCAAACAAA
TGGTCGTGCTGTATTCTGTGCAGCACCACAGTTGCTTCCTCTACGTGGGT
TCCATACTGGTGGACGAGTTCGCAAAGTCCAGCGAGTGCATTGGCGGACT
TTTGGAGATGCTTCAGGCATTTATAGAGCCCACATTTGGCCTGCTTCAGA
TGGAGAACGGTCTAAAAAATAACCCCGACACAGTGGATGACTTCTTCCGT
TTGGCATCGCGTTACCTGGACTGTTGTCCCCACCAGCTGCTCCAGAGCAG
TCTCATCACGCCGATCTTCCAGTGTGCGCTAATAGCCTGCTCCTTGGACC
ACCGCGAGGCCAACTCGTCTGTGATGAAGTTCTTTATCAACCTGTTGGTT
TGGGGCAGATCGAATAACCATAGCCGGAACACCGAGTGCCGTCCCTTGGT
GGTGGAGCTGGCCAGTCAGCATGGCGGTGCTCTGGTGATGAACCTGATCC
AGGCATCCGTCTTTCAGCTGCACT
hide Associated Information
Gene(s)
(targeted or local)
Allele(s)
Transcripts(s)
Polypeptide(s)
Construct
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PCR template
genomic
(Perkins et al., 1.15.2009)
Primer 1
AGTCTAGATGGTGAGAAAGCAAGCAATG
(Perkins et al., 1.15.2009)
Primer 2
AAGAATTCAGTGCAGCTGAAAGACGGAT
(Perkins et al., 1.15.2009)
Comments
hide Collection Information
hide Collection: TRiP-1
Symbol TRiP-1
Source and Progenitors
Species of derivation D. melanogaster  
Strain of derivation
Vector or progenitor construct
Description
Collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene; use a targeted insertion site.
(Perkins et al., 1.15.2009)
Additional data
The approach used by the TRiP is to generate transgenic animals with an RNAi hairpin under UAS-GAL4 control. The hairpin-containing transgenes are inserted via site-specific recombination into genomic loci known to be optimal for expression. This specific collection was constructed in the pVALIUM10 vector and inserted into the P{CaryP}attP2 target element.
(Perkins et al., 1.15.2009)
Cell lines
Observed in
Not observed in
hide Comments
hide Stocks Listed in FlyBase ( 1 )
Bloomington
25988
y1 v1; P{TRiP.JF02010}attP2
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hide Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym
dsRNA-JF02010
 
JF02010
(Perkins et al., 1.15.2009)
Secondary FlyBase IDs
    hide References ( 1 )
    Personal communication to FlyBase
    Perkins et al., 1.15.2009, Initial TRiP stock collection.
    Initial TRiP stock collection. [FBrf0206489]