A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Sequence Feature: RNAi_reagent

General Information
Symbol dsRNA-KK100711 Species D. melanogaster
Feature type RNAi_reagent FlyBase ID FBsf0000086824
Collection VDRC-2   Associated gene(s) CG14621
Genomic Location
Chromosome (arm) X Sequence location X:21,848,506..21,848,900 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBtr0342876 FBtr0339557 FBtr0339558 FBtr0077344 FBtr0339499 FBtr0077369 FBsf0000024677 FBsf0000020006 FBsf0000019998 FBsf0000074160 FBsf0000086824 FBsf0000089036 FBsf0000077573 FBsf0000399506 FBsf0000404011 FBsf0000398588 FBsf0000398581
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
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This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates Click here to see a list of all updates to this record from FB2010_08 and on.
hide Sequence Data
Length 395
Comments
One copy of repeat shown.
(Keleman et al., 2009.8.5)
Sequence 
TTGTGATCGCAGTATCGCTCCTAATCCTGGGCAACCCAGTGACGTGGGTC
AATTGCGTAGGCATGACGCTGGCCATTGTGGGTGTGCTCTGCTACAATCG
CGCCAAACAGCTTACGCGGGGTCGCGAGCAACCGACACTGCCGCTGTCGC
AGACTAGCTATGTCAAGTACTCGCCGCTGGAGCAACAGGCAGATCCGTAC
TACCGGGGATCGGTCAACGGGAAGCTATCCAATGGCTTGCACAGAACGCC
CGGAAACAGTCTGCTGGCGAATGGCAGCGGAATGCCGAGCGGGACGGCAA
CGCGGCTACTATTTGTTTAGTGTGTAGTTAGTGAATGTATCCAATAAGCG
GGTTAGCTAAATAGTATTAACCTGTGACGACCACCATCCAAATTC
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Gene(s)
(targeted or local)
Allele(s)
Transcripts(s)
Polypeptide(s)
Construct
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PCR template
genomic
(Keleman et al., 2009.8.5)
Primer 1
TTGTGATCGCAGTATCGCTC
(Keleman et al., 2009.8.5)
Primer 2
GAATTTGGATGGTGGTCGTC
(Keleman et al., 2009.8.5)
Comments
hide Collection Information
hide Collection: VDRC-2
Symbol VDRC-2
Source and Progenitors
Species of derivation D. melanogaster  
Strain of derivation
Vector or progenitor construct
Description
Collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene; use a targeted insertion site.
(Keleman et al., 2009.8.5)
Additional data
The phiC31 RNAi library (KK) was created using the phiC31 integrase system to target RNAi transgenes to a specific landing site on the second chromosome. This insertion site (P{attP,y[+],w[3']}VIE-260B) was selected based on its low level of basal expression and consistent high level of GAL4-dependent expression across a range of different tissues. The RNAi transgenes consist of inverted repeats that were designed to reduce the risk of off-target effects.
(Keleman et al., 2009.8.5)
Cell lines
Observed in
Not observed in
hide Comments
hide Stocks Listed in FlyBase ( 1 )
VDRC
v106983
P{KK100711}VIE-260B
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hide Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym
100711
(Keleman et al., 2009.8.5)
dsRNA-KK100711
 
Secondary FlyBase IDs
    hide References ( 1 )
    Personal communication to FlyBase
    Keleman et al., 2009.8.5, RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center
    RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0208510]