A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Sequence Feature: RNAi_reagent

General Information
Symbol dsRNA-KK106891 Species D. melanogaster
Feature type RNAi_reagent FlyBase ID FBsf0000090518
Collection VDRC-2   Associated gene(s) Ir56b
Genomic Location
Chromosome (arm) 2R Sequence location 2R:15,395,761..15,396,185 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBtr0086498 FBtr0086465 FBtr0086466 FBsf0000006289 FBsf0000026319 FBsf0000023820 FBsf0000006290 FBsf0000090518 FBsf0000072751 FBsf0000411790 FBsf0000405250 FBsf0000388601 FBsf0000388602
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
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FB2013_03
FB2013_02
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hide Sequence Data
Length 425
Comments
One copy of repeat shown.
(Keleman et al., 2009.8.5)
Sequence 
GTTTGTGGTGCCCAAGTTCTGTGGTCCCTACATGGAGATAGTCAAGCACT
TCGCTGAGGTGTACCACTACCAGCTGTTTCTTGATTCTCTCGAGAGTTTG
CCCAAGAAATCGGTGGTGGAACAGGACATTATTAGCGGCAAGTACAACCT
CTCGCTCCATGGGGTTATCATTCGGCCAGAGGAAACCAGTGACTTCTTCA
ACGCCACGCAGCATAGTTATCCGCTGGAGCTGATGACGAACTGCGTTATG
GTACCCCTGGCTCCCGAGCTGCCCAAGTGGATGTACATGGTGTGGCCGCT
GGGCAAGTACATCTGGACATGCCTCTTCCTCGGCACCTTCTATGTCGCGC
TCCTGCTGAGATATGTGCACTGGCGGGAGCCGGGGAATGCCACCCGATCA
TACACCAGGAATGTGCTTCACGCTA
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Gene(s)
(targeted or local)
Allele(s)
Transcripts(s)
Polypeptide(s)
Construct
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PCR template
genomic
(Keleman et al., 2009.8.5)
Primer 1
GTTTGTGGTGCCCAAGTTCT
(Keleman et al., 2009.8.5)
Primer 2
TAGCGTGAAGCACATTCCTG
(Keleman et al., 2009.8.5)
Comments
hide Collection Information
hide Collection: VDRC-2
Symbol VDRC-2
Source and Progenitors
Species of derivation D. melanogaster  
Strain of derivation
Vector or progenitor construct
Description
Collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene; use a targeted insertion site.
(Keleman et al., 2009.8.5)
Additional data
The phiC31 RNAi library (KK) was created using the phiC31 integrase system to target RNAi transgenes to a specific landing site on the second chromosome. This insertion site (P{attP,y[+],w[3']}VIE-260B) was selected based on its low level of basal expression and consistent high level of GAL4-dependent expression across a range of different tissues. The RNAi transgenes consist of inverted repeats that were designed to reduce the risk of off-target effects.
(Keleman et al., 2009.8.5)
Cell lines
Observed in
Not observed in
hide Comments
hide Stocks Listed in FlyBase ( 1 )
VDRC
v105928
P{KK106891}VIE-260B
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hide Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym
106891
(Keleman et al., 2009.8.5)
dsRNA-KK106891
 
Secondary FlyBase IDs
    hide References ( 1 )
    Personal communication to FlyBase
    Keleman et al., 2009.8.5, RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center
    RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0208510]