A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Sequence Feature: RNAi_reagent

General Information
Symbol dsRNA-KK107040 Species D. melanogaster
Feature type RNAi_reagent FlyBase ID FBsf0000090629
Collection VDRC-2   Associated gene(s) Dpt
Genomic Location
Chromosome (arm) 2R Sequence location 2R:14,753,412..14,753,830 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBtr0304818 FBtr0307055 FBtr0340446 FBtr0086620 FBtr0086621 FBtr0304896 FBsf0000005512 FBsf0000007146 FBsf0000032259 FBsf0000005752 FBsf0000032260 FBsf0000032261 FBsf0000005513 FBsf0000075457 FBsf0000090629 FBsf0000093983 FBsf0000095017 FBsf0000405389 FBsf0000410158 FBsf0000411345 FBsf0000387981 FBsf0000389414 FBsf0000388096 FBsf0000387982
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
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FB2013_03
FB2013_02
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hide Sequence Data
Length 419
Comments
One copy of repeat shown.
(Keleman et al., 2009.8.5)
Sequence 
ACCGCAGTACCCACTCAATCTTCAGGGAGGCGGCGGTGGCCAGAGCGGCG
ATGGTTTTGGCTTTGCAGTCCAGGGTCACCAGAAGGTGTGGACCAGCGAC
AATGGACGCCACGAGATTGGACTGAATGGAGGATATGGACAGCACTTGGG
AGGACCATATGGCAACTCAGAACCGAGCTGGAAAGTGGGAAGCACCTACA
CCTACAGATTTCCGAATTTCTAAGCTTCATAAATATTTTATTGTAAAAAA
CTTCACCAAATATTATCTCGATTGGTATCCGAGTCTAGCTATTATAAAAA
CCATACCCACTTTGTATATTCAGATAATTGCAAAATATATAACCGAATAC
AACGATATGCATACCTGACCATAATTTCACTATCTCAAATGCCATCAATG
TCCTTGGCGTTGTTTGTTT
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Gene(s)
(targeted or local)
Allele(s)
Transcripts(s)
Polypeptide(s)
Construct
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hide Progenitors
PCR template
genomic
(Keleman et al., 2009.8.5)
Primer 1
ACCGCAGTACCCACTCAATC
(Keleman et al., 2009.8.5)
Primer 2
AAACAAACAACGCCAAGGAC
(Keleman et al., 2009.8.5)
Comments
hide Collection Information
hide Collection: VDRC-2
Symbol VDRC-2
Source and Progenitors
Species of derivation D. melanogaster  
Strain of derivation
Vector or progenitor construct
Description
Collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene; use a targeted insertion site.
(Keleman et al., 2009.8.5)
Additional data
The phiC31 RNAi library (KK) was created using the phiC31 integrase system to target RNAi transgenes to a specific landing site on the second chromosome. This insertion site (P{attP,y[+],w[3']}VIE-260B) was selected based on its low level of basal expression and consistent high level of GAL4-dependent expression across a range of different tissues. The RNAi transgenes consist of inverted repeats that were designed to reduce the risk of off-target effects.
(Keleman et al., 2009.8.5)
Cell lines
Observed in
Not observed in
hide Comments
hide Stocks Listed in FlyBase ( 1 )
VDRC
v104287
P{KK107040}VIE-260B
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hide Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym
107040
(Keleman et al., 2009.8.5)
dsRNA-KK107040
 
Secondary FlyBase IDs
    hide References ( 1 )
    Personal communication to FlyBase
    Keleman et al., 2009.8.5, RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center
    RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0208510]