A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Sequence Feature: RNAi_reagent

General Information
Symbol dsRNA-KK107079 Species D. melanogaster
Feature type RNAi_reagent FlyBase ID FBsf0000090658
Collection VDRC-2   Associated gene(s) Fad2
Genomic Location
Chromosome (arm) 3L Sequence location 3L:11,016,710..11,017,041 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBtr0076196 FBtr0300188 FBsf0000028559 FBsf0000010789 FBsf0000028560 FBsf0000021871 FBsf0000073045 FBsf0000090658 FBsf0000405427 FBsf0000392010
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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hide Sequence Data
Length 332
Comments
One copy of repeat shown.
(Keleman et al., 2009.8.5)
Sequence 
GTCGACGGAGCTAAGCAATCGGAAGACCACCGATGGAAGTAAACTGGAGT
TGGTATGGGTCAATATAGTGCTGTTCGTGATCCTTCACATATCTTCGCTA
TATGGAGTCTGGCTGCTCTTCACTTCGGCCACGTGGACAACGGTCGTGCT
CTTTTGGCCAACGGTTGTGGTGACCATTTTGGGAGTTTCCGGCGGTGCCC
ATCGTTTGTGGGCTCATCGCACATTCAAGGCGAATACACCGCTCAAGCTG
ATCTTTTTGTTCCTGAACACTTTGGCCTTTCAGGATGCGGTCTACTATTG
GGCCCGGGATCATCGCGTCCACCACAAGTATA
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Gene(s)
(targeted or local)
Allele(s)
Transcripts(s)
Polypeptide(s)
Construct
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PCR template
genomic
(Keleman et al., 2009.8.5)
Primer 1
GTCGACGGAGCTAAGCAATC
(Keleman et al., 2009.8.5)
Primer 2
TATACTTGTGGTGGACGCGA
(Keleman et al., 2009.8.5)
Comments
hide Collection Information
hide Collection: VDRC-2
Symbol VDRC-2
Source and Progenitors
Species of derivation D. melanogaster  
Strain of derivation
Vector or progenitor construct
Description
Collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene; use a targeted insertion site.
(Keleman et al., 2009.8.5)
Additional data
The phiC31 RNAi library (KK) was created using the phiC31 integrase system to target RNAi transgenes to a specific landing site on the second chromosome. This insertion site (P{attP,y[+],w[3']}VIE-260B) was selected based on its low level of basal expression and consistent high level of GAL4-dependent expression across a range of different tissues. The RNAi transgenes consist of inverted repeats that were designed to reduce the risk of off-target effects.
(Keleman et al., 2009.8.5)
Cell lines
Observed in
Not observed in
hide Comments
hide Stocks Listed in FlyBase ( 1 )
VDRC
v101190
P{KK107079}VIE-260B
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hide Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym
107079
(Keleman et al., 2009.8.5)
dsRNA-KK107079
 
Secondary FlyBase IDs
    hide References ( 1 )
    Personal communication to FlyBase
    Keleman et al., 2009.8.5, RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center
    RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0208510]