A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Sequence Feature: RNAi_reagent

General Information
Symbol dsRNA-KK108638 Species D. melanogaster
Feature type RNAi_reagent FlyBase ID FBsf0000091670
Collection VDRC-2   Associated gene(s) Det
Genomic Location
Chromosome (arm) 3R Sequence location 3R:12,975,972..12,976,179 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBtr0337043 FBtr0083414 FBtr0083408 FBtr0083409 FBtr0083410 FBtr0337042 FBsf0000013983 FBsf0000015249 FBsf0000015250 FBsf0000023896 FBsf0000091670 FBsf0000083740 FBsf0000087678 FBsf0000195036 FBsf0000079321 FBsf0000428122 FBsf0000428110 FBsf0000406914 FBsf0000401071 FBsf0000405944 FBsf0000393668 FBsf0000394855 FBsf0000394856
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
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FB2013_03
FB2013_02
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hide Sequence Data
Length 208
Comments
One copy of repeat shown.
(Keleman et al., 2009.8.5)
Sequence 
TCTGGAAATTCTTGGAACCGTCGTTAAAGGCAGCATAGAGAAAACCTGCA
AAGCCTTCAAATCGAGCTTCGTTCGGGAGAATGAGAAGCGTCTAGATGAG
TTTACGCGTAATCAAAAATAGAGCGCTAATTTTTAAACCTTAAATATACA
TATATAAAACTCGCTATTTATCAAGATTTTTAATAAAACGCAATGTTAGT
CCATCGAA
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Gene(s)
(targeted or local)
Allele(s)
Transcripts(s)
Polypeptide(s)
Construct
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PCR template
genomic
(Keleman et al., 2009.8.5)
Primer 1
TCTGGAAATTCTTGGAACCG
(Keleman et al., 2009.8.5)
Primer 2
TTCGATGGACTAACATTGCG
(Keleman et al., 2009.8.5)
Comments
hide Collection Information
hide Collection: VDRC-2
Symbol VDRC-2
Source and Progenitors
Species of derivation D. melanogaster  
Strain of derivation
Vector or progenitor construct
Description
Collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene; use a targeted insertion site.
(Keleman et al., 2009.8.5)
Additional data
The phiC31 RNAi library (KK) was created using the phiC31 integrase system to target RNAi transgenes to a specific landing site on the second chromosome. This insertion site (P{attP,y[+],w[3']}VIE-260B) was selected based on its low level of basal expression and consistent high level of GAL4-dependent expression across a range of different tissues. The RNAi transgenes consist of inverted repeats that were designed to reduce the risk of off-target effects.
(Keleman et al., 2009.8.5)
Cell lines
Observed in
Not observed in
hide Comments
hide Stocks Listed in FlyBase ( 1 )
VDRC
v105741
P{KK108638}VIE-260B
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hide Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym
108638
(Keleman et al., 2009.8.5)
dsRNA-KK108638
 
Secondary FlyBase IDs
    hide References ( 1 )
    Personal communication to FlyBase
    Keleman et al., 2009.8.5, RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center
    RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0208510]