A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Sequence Feature: RNAi_reagent

General Information
Symbol dsRNA-KK108812 Species D. melanogaster
Feature type RNAi_reagent FlyBase ID FBsf0000091806
Collection VDRC-2   Associated gene(s) CG4169
Genomic Location
Chromosome (arm) 3L Sequence location 3L:16,578,445..16,578,787 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBtr0075308 FBtr0075309 FBtr0331557 FBtr0075365 FBtr0075310 FBsf0000029144 FBsf0000010896 FBsf0000026152 FBsf0000029143 FBsf0000026472 FBsf0000010032 FBsf0000034053 FBsf0000010029 FBsf0000021028 FBsf0000025319 FBsf0000009096 FBsf0000026297 FBsf0000020606 FBsf0000079752 FBsf0000091806 FBsf0000084638 FBsf0000087323 FBsf0000073123 FBsf0000090335 FBsf0000075738 FBsf0000086216 FBsf0000401398 FBsf0000407081 FBsf0000400333 FBsf0000404989 FBsf0000392112 FBsf0000391278 FBsf0000391275 FBsf0000390535 FBsf0000432402 FBsf0000431956
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Description
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FB2013_03
FB2013_02
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hide Sequence Data
Length 343
Comments
One copy of repeat shown.
(Keleman et al., 2009.8.5)
Sequence 
GAATCGACAACAACACGCTGGCCGGCTTCGCCCAGACCCTGCAGTTCCCC
AGCGGCGGCAGCAAGGCTGCCTCCGCCAACTGGTATGGTGGAGATGCGCG
CAAGGACACTTCTGGTCACCGTGCGGTCGTCGCCGTTGCTGGACAGGGCG
CCGCCGCATCGAACCACAAGGAAGCGCTGGCATTCGCCATCCTGGAGCAG
GCGCTCGGCGCCAAGGCCGCCACCAAGCGCGGCACTTCTGCTGGACTCTT
TGGCGAGGCCGTCAACTGCGCCGGCGGCGTCGGAGCCTCTGTCAAGGCTG
TGAATGCCAGCTACTCGGACGCTGGTCTGTTCGGTTTTGTGGT
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Gene(s)
(targeted or local)
Allele(s)
Transcripts(s)
Polypeptide(s)
Construct
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PCR template
genomic
(Keleman et al., 2009.8.5)
Primer 1
GAATCGACAACAACACGCTG
(Keleman et al., 2009.8.5)
Primer 2
ACCACAAAACCGAACAGACC
(Keleman et al., 2009.8.5)
Comments
hide Collection Information
hide Collection: VDRC-2
Symbol VDRC-2
Source and Progenitors
Species of derivation D. melanogaster  
Strain of derivation
Vector or progenitor construct
Description
Collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene; use a targeted insertion site.
(Keleman et al., 2009.8.5)
Additional data
The phiC31 RNAi library (KK) was created using the phiC31 integrase system to target RNAi transgenes to a specific landing site on the second chromosome. This insertion site (P{attP,y[+],w[3']}VIE-260B) was selected based on its low level of basal expression and consistent high level of GAL4-dependent expression across a range of different tissues. The RNAi transgenes consist of inverted repeats that were designed to reduce the risk of off-target effects.
(Keleman et al., 2009.8.5)
Cell lines
Observed in
Not observed in
hide Comments
hide Stocks Listed in FlyBase ( 1 )
VDRC
v100818
P{KK108812}VIE-260B
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hide Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym
108812
(Keleman et al., 2009.8.5)
dsRNA-KK108812
 
Secondary FlyBase IDs
    hide References ( 1 )
    Personal communication to FlyBase
    Keleman et al., 2009.8.5, RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center
    RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0208510]