A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Sequence Feature: RNAi_reagent

General Information
Symbol dsRNA-KK110727 Species D. melanogaster
Feature type RNAi_reagent FlyBase ID FBsf0000093003
Collection VDRC-2   Associated gene(s) fd3F
Genomic Location
Chromosome (arm) X Sequence location X:3,704,264..3,704,534 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBtr0335187 FBtr0112801 FBsf0000017276 FBsf0000032287 FBsf0000021460 FBsf0000032288 FBsf0000074654 FBsf0000093003 FBsf0000408831 FBsf0000396448
hide Recent Updates
Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
Update Feed
Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
atom feed
FB2013_03
FB2013_02
All updates Click here to see a list of all updates to this record from FB2010_08 and on.
hide Sequence Data
Length 271
Comments
One copy of repeat shown.
(Keleman et al., 2009.8.5)
Sequence 
AATCCCATACACAAGGACGTGGTTGTTACGGAAAGTGTCCTACGGCAGCA
GCATGGCACCAACTTGGGCGGAGGCAATAACAACCAAAATAACCAAATGG
GCGGCAATAGCCACATGGGTGGCGGCAACTCGCAGTACTACATCACCGAA
ATTGATCCCATGGAGCTCGGCATTCAGATGTCGCACCAAGTGGAGCCCTC
CGAGGAGGAAGTACTCTTCAGCGACGAGTTCAACCTTAACTACTTTGGCT
ATAATCCGGGTAGCGATATCG
hide Associated Information
Gene(s)
(targeted or local)
Allele(s)
Transcripts(s)
Polypeptide(s)
Construct
hide Experimental Data
hide Progenitors
PCR template
genomic
(Keleman et al., 2009.8.5)
Primer 1
AATCCCATACACAAGGACGTG
(Keleman et al., 2009.8.5)
Primer 2
CGATATCGCTACCCGGATTA
(Keleman et al., 2009.8.5)
Comments
hide Collection Information
hide Collection: VDRC-2
Symbol VDRC-2
Source and Progenitors
Species of derivation D. melanogaster  
Strain of derivation
Vector or progenitor construct
Description
Collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene; use a targeted insertion site.
(Keleman et al., 2009.8.5)
Additional data
The phiC31 RNAi library (KK) was created using the phiC31 integrase system to target RNAi transgenes to a specific landing site on the second chromosome. This insertion site (P{attP,y[+],w[3']}VIE-260B) was selected based on its low level of basal expression and consistent high level of GAL4-dependent expression across a range of different tissues. The RNAi transgenes consist of inverted repeats that were designed to reduce the risk of off-target effects.
(Keleman et al., 2009.8.5)
Cell lines
Observed in
Not observed in
hide Comments
hide Stocks Listed in FlyBase ( 1 )
VDRC
v102106
P{KK110727}VIE-260B
hide External Crossreferences & Linkouts
hide Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym
110727
(Keleman et al., 2009.8.5)
dsRNA-KK110727
 
Secondary FlyBase IDs
    hide References ( 1 )
    Personal communication to FlyBase
    Keleman et al., 2009.8.5, RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center
    RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0208510]