A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Sequence Feature: RNAi_reagent

General Information
Symbol dsRNA-KK113105 Species D. melanogaster
Feature type RNAi_reagent FlyBase ID FBsf0000094657
Collection VDRC-2   Associated gene(s) TwdlD
Genomic Location
Chromosome (arm) 3R Sequence location 3R:22,465,898..22,466,187 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBtr0085035 FBsf0000014231 FBsf0000022645 FBsf0000013290 FBsf0000094657 FBsf0000075870 FBsf0000410993 FBsf0000393901
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Description
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This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
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FB2013_03
FB2013_02
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hide Sequence Data
Length 290
Comments
One copy of repeat shown.
(Keleman et al., 2009.8.5)
Sequence 
AGCTGCAGTGCCGATAAACTGGGCTACAACTACCAACCCGTGGCACATGC
CGATGAGGGTCTGTCCTTCCTGCCCGGAAGTGGTCAGGTGATTGGAGAAC
TTCCGTCGCAGGTGCTGCCCGTGCAGAGTGGCGAAGCAGTCCTCTCCCAG
CCCATTGAAGCACCTGTTGCTCCCCAGATTGCACCTTTGGTAGAGGAGTT
CCAGAAGGAGTTCTACAGCTACGCCGCCCCCGAGGAACAGTACGATGAGG
GTGCCAGCAACCAGCAGATCGCCAACTCCCTGAAAAAGAA
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Gene(s)
(targeted or local)
Allele(s)
Transcripts(s)
Polypeptide(s)
Construct
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PCR template
genomic
(Keleman et al., 2009.8.5)
Primer 1
AGCTGCAGTGCCGATAAACT
(Keleman et al., 2009.8.5)
Primer 2
TTCTTTTTCAGGGAGTTGGC
(Keleman et al., 2009.8.5)
Comments
hide Collection Information
hide Collection: VDRC-2
Symbol VDRC-2
Source and Progenitors
Species of derivation D. melanogaster  
Strain of derivation
Vector or progenitor construct
Description
Collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene; use a targeted insertion site.
(Keleman et al., 2009.8.5)
Additional data
The phiC31 RNAi library (KK) was created using the phiC31 integrase system to target RNAi transgenes to a specific landing site on the second chromosome. This insertion site (P{attP,y[+],w[3']}VIE-260B) was selected based on its low level of basal expression and consistent high level of GAL4-dependent expression across a range of different tissues. The RNAi transgenes consist of inverted repeats that were designed to reduce the risk of off-target effects.
(Keleman et al., 2009.8.5)
Cell lines
Observed in
Not observed in
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hide Stocks Listed in FlyBase ( 1 )
VDRC
v105039
P{KK113105}VIE-260B
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hide Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym
113105
(Keleman et al., 2009.8.5)
dsRNA-KK113105
 
Secondary FlyBase IDs
    hide References ( 1 )
    Personal communication to FlyBase
    Keleman et al., 2009.8.5, RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center
    RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0208510]