A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Sequence Feature: RNAi_reagent

General Information
Symbol dsRNA-JF03086 Species D. melanogaster
Feature type RNAi_reagent FlyBase ID FBsf0000095775
Collection TRiP-1   Associated gene(s) ro
Genomic Location
Chromosome (arm) 3R Sequence location 3R:22,706,486..22,706,846 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBtr0085064 FBtr0085090 FBtr0085091 FBtr0336724 FBtr0085065 FBsf0000027003 FBsf0000015307 FBsf0000016529 FBsf0000021864 FBsf0000094073 FBsf0000072220 FBsf0000095775 FBsf0000410264 FBsf0000409417 FBsf0000394913 FBsf0000396093
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates Click here to see a list of all updates to this record from FB2010_08 and on.
hide Sequence Data
Length 361
Comments
One copy of repeat shown.
(Perkins et al., 2009)
Sequence 
CGGCACATCCTCAAACGAAATGCAACGTCACAAAGTTGAGATTGGCTCAC
CCGACGGCTCGCCGGGCATCAAAAGGAGTGACAGCTTAGATCCCATAGCG
AACACTACGATTCTATCCGTGCCGCAACGTCCCTCGTCGCCGCGACAGTT
TTTCGAACGTCTGTACGGGCATTTGGAAACGCGCTCCTCGGAAAATGGGG
AAATCGACGTCGGAACACACGCCCACAAGCCGCCGCCCTGTGACACGCCC
TATCACAGCGATGGAGGAAGCGTCTCCTCGCCAGACATTTCCATCAGCGA
TGAACGCACCTCGCTAGCCGCATATCCCGCCTACGATTTCTATGGTCACG
CCAAGGACTAT
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Gene(s)
(targeted or local)
Allele(s)
Transcripts(s)
Polypeptide(s)
Construct
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PCR template
cDNA
(Perkins et al., 2009)
Primer 1
AAGAATTCGGCACATCCTCAAACGAAAT
(Perkins et al., 2009)
Primer 2
AGTCTAGAATAGTCCTTGGCGTGACCAT
(Perkins et al., 2009)
Comments
hide Collection Information
hide Collection: TRiP-1
Symbol TRiP-1
Source and Progenitors
Species of derivation D. melanogaster  
Strain of derivation
Vector or progenitor construct
Description
Collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene; use a targeted insertion site.
(Perkins et al., 1.15.2009)
Additional data
The approach used by the TRiP is to generate transgenic animals with an RNAi hairpin under UAS-GAL4 control. The hairpin-containing transgenes are inserted via site-specific recombination into genomic loci known to be optimal for expression. This specific collection was constructed in the pVALIUM10 vector and inserted into the P{CaryP}attP2 target element.
(Perkins et al., 1.15.2009)
Cell lines
Observed in
Not observed in
hide Comments
hide Stocks Listed in FlyBase ( 1 )
Bloomington
28671
y1 v1; P{TRiP.JF03086}attP2
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hide Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym
dsRNA-JF03086
 
JF03086
(Perkins et al., 2009)
Secondary FlyBase IDs
    hide References ( 1 )
    Personal communication to FlyBase
    Perkins et al., 2009, Update to TRiP stock collection.
    Update to TRiP stock collection. [FBrf0208864]