A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Sequence Feature: RNAi_reagent

General Information
Symbol dsRNA-JF01682 Species D. melanogaster
Feature type RNAi_reagent FlyBase ID FBsf0000096012
Collection TRiP-1   Associated gene(s) dome
Genomic Location
Chromosome (arm) X Sequence location X:19,572,452..19,573,017 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBtr0074756 FBsf0000029671 FBsf0000033046 FBsf0000033047 FBsf0000018985 FBsf0000089086 FBsf0000081772 FBsf0000071026 FBsf0000073230 FBsf0000169002 FBsf0000096012 FBsf0000151640 FBsf0000152022 FBsf0000404091 FBsf0000415243 FBsf0000415323 FBsf0000397717
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates Click here to see a list of all updates to this record from FB2010_08 and on.
hide Sequence Data
Length 566
Comments
One copy of repeat shown.
(Perkins et al., 2009)
Sequence 
CATCACTTCACCACGTCACCGGACCAACTGCTCACGCTGCACATGGCCGT
GTCGGCCAACTACCAATCGCATAATACGGGCCTACACTGGGCGATCTGCA
GTAGCGACAAGAAGGACGATTTGGCCAAGATGGAGCCGTCGATCGATGTG
GCCACCAGCACCTCACTGACGGTCAGTTGGTCGGAGCGCGTCTGCGCAGT
GATCCTGGCCGGCTATAATCTGACCTACTGCCAGCGCTCGGCCGGCCGTC
CGGACAATTGCACGACGGTGACCATCGATCGCTATACGAACAAGCACGTC
ATCCAGAATCTGGTGCCCTACACGGACTACAGTGTCAAGATGCTGATGTA
TTCGGACTCGCGGGTCAGCAAGTACAGCGACGAGCTGGTCAACCGCACCG
GAGAGGCGGCGCCTAGCCAGCCGCGGGAGTTGCAGCTAATCCGCGTGACC
AGCGACAGCGTGGAGCTCGCCTGGAAGCCACCGCTGCTGGCCAACGGCGT
GGTGCGCGCCTACGAGGGCACCTTCCGCTCGCTACATGACAACGTGACCG
ATACATTCCGCGTAAG
hide Associated Information
Gene(s)
(targeted or local)
Allele(s)
Transcripts(s)
Polypeptide(s)
Construct
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PCR template
cDNA
(Perkins et al., 2009)
Primer 1
CACCCATCACTTCACCACGTCACC
(Perkins et al., 2009)
Primer 2
CTTACGCGGAATGTATCGGT
(Perkins et al., 2009)
Comments
hide Collection Information
hide Collection: TRiP-1
Symbol TRiP-1
Source and Progenitors
Species of derivation D. melanogaster  
Strain of derivation
Vector or progenitor construct
Description
Collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene; use a targeted insertion site.
(Perkins et al., 1.15.2009)
Additional data
The approach used by the TRiP is to generate transgenic animals with an RNAi hairpin under UAS-GAL4 control. The hairpin-containing transgenes are inserted via site-specific recombination into genomic loci known to be optimal for expression. This specific collection was constructed in the pVALIUM10 vector and inserted into the P{CaryP}attP2 target element.
(Perkins et al., 1.15.2009)
Cell lines
Observed in
Not observed in
hide Comments
hide Stocks Listed in FlyBase ( 1 )
Bloomington
28983
y1 v1; P{TRiP.JF01682}attP2
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hide Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym
dsRNA-JF01682
 
JF01682
(Perkins et al., 2009)
Secondary FlyBase IDs
    hide References ( 1 )
    Personal communication to FlyBase
    Perkins et al., 2009, Update to TRiP stock collection.
    Update to TRiP stock collection. [FBrf0208864]