A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Sequence Feature: RNAi_reagent

General Information
Symbol dsRNA-JF03263 Species D. melanogaster
Feature type RNAi_reagent FlyBase ID FBsf0000096523
Collection TRiP-1   Associated gene(s) Or65c
Genomic Location
Chromosome (arm) 3L Sequence location 3L:6,321,401..6,322,027 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBtr0077005 FBtr0077006 FBtr0077007 FBsf0000032465 FBsf0000008487 FBsf0000020199 FBsf0000032466 FBsf0000088194 FBsf0000072975 FBsf0000081990 FBsf0000094442 FBsf0000095490 FBsf0000401989 FBsf0000410698 FBsf0000390398
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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hide Sequence Data
Length 572
Comments
One copy of repeat shown.
(Perkins et al., 2009)
Sequence 
AAGTCGATTCACCCCATCAGCCACGCATTTATCTATTTGTTTCAGACTTG
GAATGTAATGTATTTTCTGACTTGGCTTGTGTGCATTGAAGGACTCTCTG
TATCTATTTACGTGGAAATAACATTTGCCATCGAAGTTCTATGCCTCGAA
CTGCGTCACCTTCATCAGAGGTGCCATGGATATGAGCAGTTGAGATTGGA
GACGAATCGCCTGGTCCAGTTCCATCAGAAGATTGTTCACATCTTAGATC
ACACTAACAAAGTTTTTCATGGAACCCTCATAATGCAAATGGGGGTTAAC
TTTTTCCTGGTGTCCCTATCGGTTCTAGAGGCCATGGAGGCAAGAAAAGA
CCCCAAGGTGGTGGCCCAGTTTGCCGTCCTCATGCTGCTAGCCTTGGGAC
ATCTATCCATGTGGTCGTATTTTGGAGACCTGTTATCGCAGAAATCCTTG
ACAATTTCAGAGGCTGCTTATGAGGCGTATGATCCCATCAAAGGATCCAA
GGATGTTTATAGAGACCTCTGCCTAATAATTCGGCGTGGCCAGGAGCCTC
TGATTATGAGAGCCAGCCCCTT
hide Associated Information
Gene(s)
(targeted or local)
Allele(s)
Transcripts(s)
Polypeptide(s)
Construct
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hide Progenitors
PCR template
cDNA
(Perkins et al., 2009)
Primer 1
AAGAATTCAAGTCGATTCACCCCATCAG
(Perkins et al., 2009)
Primer 2
AGACTAGTAAGGGGCTGGCTCTCATAAT
(Perkins et al., 2009)
Comments
hide Collection Information
hide Collection: TRiP-1
Symbol TRiP-1
Source and Progenitors
Species of derivation D. melanogaster  
Strain of derivation
Vector or progenitor construct
Description
Collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene; use a targeted insertion site.
(Perkins et al., 1.15.2009)
Additional data
The approach used by the TRiP is to generate transgenic animals with an RNAi hairpin under UAS-GAL4 control. The hairpin-containing transgenes are inserted via site-specific recombination into genomic loci known to be optimal for expression. This specific collection was constructed in the pVALIUM10 vector and inserted into the P{CaryP}attP2 target element.
(Perkins et al., 1.15.2009)
Cell lines
Observed in
Not observed in
hide Comments
hide Stocks Listed in FlyBase ( 1 )
Bloomington
29584
y1 v1; P{TRiP.JF03263}attP2
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hide Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym
dsRNA-JF03263
 
JF03263
(Perkins et al., 2009)
Secondary FlyBase IDs
    hide References ( 1 )
    Personal communication to FlyBase
    Perkins et al., 2009, Update to TRiP stock collection.
    Update to TRiP stock collection. [FBrf0208864]