A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Sequence Feature: RNAi_reagent

General Information
Symbol dsRNA-JF02196 Species D. melanogaster
Feature type RNAi_reagent FlyBase ID FBsf0000096700
Collection TRiP-1   Associated gene(s) mirr
Genomic Location
Chromosome (arm) 3L Sequence location 3L:12,701,963..12,702,418 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBtr0075911 FBtr0075912 FBtr0330077 FBsf0000010990 FBsf0000033736 FBsf0000096700 FBsf0000413290 FBsf0000392203
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Length 456
Comments
One copy of repeat shown.
(Perkins et al., 2009)
Sequence 
AATATCCAGCAACTCCACCGCCTCGTCATCCAGTTCGCACTCCGGCAAGA
TATCGCCCGGCGTGAATGTGACCTCGCTGAGTGCAAAGCCATGACATCCA
TCGGCGGCATCTCCGGATCCGGAACTGGGTGCGGCCCCAATTGCCTCGCC
CATCTCCGCCTTCCGCTCGCTGATCGCCTTCCGGGAGCAGCAGAAGCTTA
CGCTCCTGCGGCCGTAGTCACTCCCAGTTTCGAAACCCATTTCGATTTCG
ATTCCGATTCCGATTCAGATGCAGATTTCGAGGCCCGGGCTTGAAAGGTA
TAATTACACTTTCGGACAATAATTTACAATGTGATTATTCGGTATTTTGA
TTAACGCCGCTTATACCAAATAGCAAGCAAACGACAAACAAATATAACCA
GCTGATAATCAGCAGCGGTTCCCCTTTGGCATGGGCCCCCAAAAACAAAA
ATTTGA
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Gene(s)
(targeted or local)
Allele(s)
Transcripts(s)
Polypeptide(s)
Construct
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PCR template
cDNA
(Perkins et al., 2009)
Primer 1
AGTCTAGAAATATCCAGCAACTCCACCG
(Perkins et al., 2009)
Primer 2
AAGAATTCAAATTTTTGTTTTTGGGGGC
(Perkins et al., 2009)
Comments
hide Collection Information
hide Collection: TRiP-1
Symbol TRiP-1
Source and Progenitors
Species of derivation D. melanogaster  
Strain of derivation
Vector or progenitor construct
Description
Collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene; use a targeted insertion site.
(Perkins et al., 1.15.2009)
Additional data
The approach used by the TRiP is to generate transgenic animals with an RNAi hairpin under UAS-GAL4 control. The hairpin-containing transgenes are inserted via site-specific recombination into genomic loci known to be optimal for expression. This specific collection was constructed in the pVALIUM10 vector and inserted into the P{CaryP}attP2 target element.
(Perkins et al., 1.15.2009)
Cell lines
Observed in
Not observed in
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hide Stocks Listed in FlyBase ( 1 )
Bloomington
31907
y1 v1; P{TRiP.JF02196}attP2
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hide Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym
dsRNA-JF02196
 
JF02196
(Perkins et al., 2009)
Secondary FlyBase IDs
    hide References ( 1 )
    Personal communication to FlyBase
    Perkins et al., 2009, Update to TRiP stock collection.
    Update to TRiP stock collection. [FBrf0208864]