Sequence Feature: exon_junction

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General Information
Symbol	Dmel:r5:3L:333640:333942:-	Species	D. melanogaster
Feature type	exon_junction	FlyBase ID	FBsf0000111616
Collection	BCM_1_RNAseq_junctions modENCODE_mRNA-Seq_EXTRA_junctions	Associated gene(s)	mthl10
Genomic Location
Chromosome (arm)	3L	Sequence location	3L:333,640..333,942 [-]
Map ( GBrowse )
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Sequence Data
Length
Comments	GT..AG
Sequence
Associated Information
Gene(s) (targeted or local)
Allele(s)
Transcripts(s)
Polypeptide(s)
Construct
Experimental Data
RNA-Seq Read Counts
Library: BCM_1_RNAseq_junctions
BCM_1_RNAseq_junctions	Expression stage(s) Read Count embryonic stage 4 -- 9 Comment:2-4hr 0 embryonic stage 4 -- 16 Comment:2-16 hr 1 embryonic stage 4 -- 16 Comment:2-16 hr 0 embryonic stage 16 Comment:14-16 hr 3 second instar larval stage -- third instar larval stage Comment:48 to 120 hrs post egg-lay 6 late third instar larval stage Comment:wandering 7 late third instar larval stage Comment:wandering 5 pupal stage Comment:aged 1 to 5 days after collection as third instar larvae 9 pupal stage P6 -- pharate adult stage P10 Comment:aged 72 hrs after collection as third instar larvae 10 adult stage Comment:aged 17 days post-eclosion 2 adult stage \| female Comment:aged 2-3 days post-eclosion 3 adult stage \| male Comment:aged 2-3 days post-eclosion 1 Total 47
Library: modENCODE_mRNA-Seq_EXTRA_junctions
modENCODE_mRNA-Seq_EXTRA_junctions	Expression stage(s) Read Count embryonic stage 1 -- 4 Comment:0-2 hr 5 embryonic stage 4 -- 9 Comment:2-4 hr 8 embryonic stage 9 -- 11 Comment:4-6 hr 16 embryonic stage 11 -- 12 Comment:6-8 hr 31 embryonic stage 12 -- 13 Comment:8-10 hr 30 embryonic stage 13 -- 15 Comment:10-12 hr 67 embryonic stage 15 -- 16 Comment:12-14 hr 69 embryonic stage 16 Comment:14-16 hr 79 embryonic stage 17(i) Comment:16-18 hr 311 embryonic stage 17(ii) Comment:18-20 hr 206 embryonic stage 17(iii) Comment:20-22 hr 120 embryonic stage 17(iv) Comment:22-24 hr 211 first instar larval stage 111 second instar larval stage 132 early third instar larval stage Comment:12 hr post-molt 43 third instar - uncleared gut stage Comment:indicated as puff stage 1-2 82 third instar - partially cleared gut stage Comment:indicated as puff stage 3-6 108 third instar - cleared gut stage Comment:indicated as puff stage 7-9 180 prepupal stage P1 317 pupal stage P5 Comment:aged 12 hrs after collection as white prepupae 448 pupal stage P6 Comment:aged 24 hrs after collection as white prepupae 224 pharate adult stage P8 Comment:aged 2 days after collection as white prepupae 112 pharate adult stage P9 -- P10 Comment:aged 3 days after collection as white prepupae 73 pharate adult stage P15 Comment:aged 4 days after collection as white prepupae 213 day 1 of adulthood \| female Comment:aged 1 day after eclosion 76 day 1 of adulthood \| male Comment:aged 1 day after eclosion 81 day 5 of adulthood \| female Comment:aged 5 days after eclosion 69 day 5 of adulthood \| male Comment:aged 5 days after eclosion 141 day 30 of adulthood \| female Comment:aged 30 days after eclosion 48 day 30 of adulthood \| male Comment:aged 30 days after eclosion 97 Total 3708
Progenitors
PCR template
Primer 1
Primer 2
Comments
Collection Information
Collection: BCM_1_RNAseq_junctions
Symbol	BCM_1_RNAseq_junctions
Source and Progenitors
Species of derivation	D. melanogaster
Strain of derivation

Vector or progenitor construct
Description
	Umbrella record for 12 collections that differ by developmental stage, from embryos to aged adults. RNA junctions identified and characterized (presented in GBrowse and in RNA junction reports). (Daines et al., 2011.3.30)
Additional data
	See report for BCM_1_RNAseq. (Daines et al., 2011.3.30)
Collection: modENCODE_mRNA-Seq_EXTRA_junctions
Symbol	modENCODE_mRNA-Seq_EXTRA_junctions
Source and Progenitors
Species of derivation	D. melanogaster
Strain of derivation

Vector or progenitor construct
Description
	Additional low confidence modENCODE junctions identified by RNA-Seq analysis of cDNA prepared from polyadenylated RNA. (Graveley, 2011.11.3)
Additional data
	See modENCODE_mRNA-Seq_U for details. (Graveley, 2011.11.3)
Cell lines
Observed in
Not observed in
Comments
	Matches FlyBase Annotated Junction.
Stocks Listed in FlyBase ( 0 )

External Crossreferences & Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	3L:333640-333941 (Daines et al., 2010.2.8) chr3L_333641_333941 (Graveley, 2011.11.3) Dmel:r5:3L:333640:333942:- (FlyBase, 1992-)
Secondary FlyBase IDs

References ( 3 )
Personal communication to FlyBase	Graveley, 2011.11.3, Additional low confidence modENCODE junctions. Additional low confidence modENCODE junctions. [FBrf0217580] Daines et al., 2010.2.8, Drosophila melanogaster mRNA high-throughput sequencing Drosophila melanogaster mRNA high-throughput sequencing [FBrf0210193]
FlyBase analysis	FlyBase, 1992-, FlyBase curation. FlyBase curation. [FBrf0105495]