A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Sequence Feature: RNAi_reagent

General Information
Symbol dsRNA-JF01299 Species D. melanogaster
Feature type RNAi_reagent FlyBase ID FBsf0000151669
Collection TRiP-2   Associated gene(s) Nurf-38
Genomic Location
Chromosome (arm) 2R Sequence location 2R:20,447,187..20,447,655 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBtr0290219 FBtr0300186 FBtr0300187 FBtr0305985 FBtr0273253 FBtr0072343 FBtr0072307 FBsf0000028754 FBsf0000024442 FBsf0000004464 FBsf0000028753 FBsf0000033523 FBsf0000087844 FBsf0000072319 FBsf0000382749 FBsf0000401342 FBsf0000387540
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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hide Sequence Data
Length 414
Comments
One copy of repeat shown.
(Ni et al., 2008)
Sequence 
ATCTGGAACTACGGAGCCCTGCCCCAGACCTGGGAAAACCCCGACCACAT
CGAGCCCTCCACCGGCTGCAAGGGTGACAACGATCCCATCGATGTCATCG
AGATTGGCTACCGAGTGGCTAAGCGCGGCGACGTGCTGAAGGTCAAGGTG
CTGGGCACCATTGCCTTGATTGACGAGGGCGAAACCGACTGGAAGATAAT
CGCCATCGATGTGAACGATCCGTTGGCCTCGAAAGTCAATGATATCGCCG
ATGTGGACCAGTACTTCCCGGGTCTGCTTCGTGCCACCGTTGAGTGGTTC
AAGATCTACAAGATCCCCGATGGCAAGCCTGAGAATCAGTTTGCTTTCAA
CGGCGATGCCAAGAATGCGGACTTTGCCAACACCATCATTGCCGAGACCC
ACAAGTTCTGGCAG
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Gene(s)
(targeted or local)
Allele(s)
Transcripts(s)
Polypeptide(s)
Construct
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PCR template
cDNA
(Ni et al., 2008)
Primer 1
GTCTAGAATCTGGAACTACGGAGCCCT
(Ni et al., 2008)
Primer 2
AGAATTCCTGCCAGAACTTGTGGGTCT
(Ni et al., 2008)
Comments
hide Collection Information
hide Collection: TRiP-2
Symbol TRiP-2
Source and Progenitors
Species of derivation D. melanogaster  
Strain of derivation
Vector or progenitor construct
Description
Collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene; use a targeted insertion site.
(Perkins et al., 2010.7.26)
Additional data
The approach used by the TRiP is to generate transgenic animals with an RNAi hairpin under UAS-GAL4 control. The hairpin-containing transgenes are inserted via site-specific recombination into genomic loci known to be optimal for expression. This specific collection was constructed in the pVALIUM1 vector and inserted into the P{CaryP}attP2 target element.
(Perkins et al., 2010.7.26)
Cell lines
Observed in
Not observed in
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hide Stocks Listed in FlyBase ( 1 )
Bloomington
31341
y1 v1; P{TRiP.JF01299}attP2
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hide Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym
dsRNA-JF01299
 
JF01299
(Ni et al., 2008)
Secondary FlyBase IDs
    hide References ( 1 )
    Research paper
    Ni et al., 2008, Nat. Methods 5(1): 49--51
    Vector and parameters for targeted transgenic RNA interference in Drosophila melanogaster. [FBrf0200406]