A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Sequence Feature: RNAi_reagent

General Information
Symbol dsRNA-11315R Species D. melanogaster
Feature type RNAi_reagent FlyBase ID FBsf0000429017
Collection NIG_RNAi_Fly-1   Associated gene(s) Npc2h
Genomic Location
Chromosome (arm) 3R Sequence location 3R:26,571,517..26,571,979 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBtr0085700 FBtr0085723 FBtr0306293 FBtr0306292 FBtr0085721 FBtr0085722 FBtr0306291 FBsf0000013790 FBsf0000013791 FBsf0000094155 FBsf0000083166 FBsf0000072376 FBsf0000094018 FBsf0000082966 FBsf0000076159 FBsf0000410362 FBsf0000410199 FBsf0000393489 FBsf0000393490 FBsf0000429017
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
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FB2013_03
FB2013_02
All updates Click here to see a list of all updates to this record from FB2010_08 and on.
hide Sequence Data
Length 463
Comments
One copy of repeat shown.
(Japanese National Institute of Genetics, 2012.5.21)
Sequence 
TTGCGTCTGAGTTCGCTCCTGCCTGTCGCCTTTGCTCTGGTGTTGTCATC
CGTCTCCGCTGAGATAGTCAACTTTCAGACTTGCGAAGATAGTGTGGACT
CCTGCTCGATTAGTCAGGTGCGGGTGACGCCCTGTCCGGAAGCCAATGCC
AATGCCGCATGCCACATTCGCCGGAGGCACCGCTTCACGATGAGCTTCGA
CTTTACGCCCCACTTCGATGCGGACACCCTGGTGGCCAGCTTGGGCTGGG
CCAAGAGCGAGAACGTGGAGCTGCCCCTGCTTACCATGGACCAGGAGGCC
TGCAAGTACACCACCTGCCCCGTGAGATCCGGAGTCACCCAGACCTACAC
CAACAACATGCCCGCCGACGCCAGGTTCCCGCTGAGTCCATACACCATCC
GTTGGGCCCTGAAGGATCCGGTTTCCCAGAAACGTTGCTGCTTCACCATC
GACATCAAGGTGG
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Gene(s)
(targeted or local)
Allele(s)
Transcripts(s)
Polypeptide(s)
Construct
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PCR template
cDNA
(Japanese National Institute of Genetics, 2012.5.21)
Primer 1
aaggcctacatggccggaccgTTGCGTCTGAGTTCGCTCCTG
(Japanese National Institute of Genetics, 2012.5.21)
Primer 2
aatctagaggtaccCCACCTTGATGTCGATGGTGA
(Japanese National Institute of Genetics, 2012.5.21)
Comments
hide Collection Information
hide Collection: NIG_RNAi_Fly-1
Symbol NIG_RNAi_Fly-1
Source and Progenitors
Species of derivation D. melanogaster  
Strain of derivation
Vector or progenitor construct
Description
Comprehensive collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene.
(Japanese National Institute of Genetics, 2012.5.21)
Additional data
A collection of over 6,400 RNAi transgenes (over 12,300 lines), each designed to target a single protein-coding gene. Each construct contains a pair of identical 500-bp amplicons produced using cDNA templates. These have been cloned as inverted repeats that are expressed using the binary GAL4/UAS system, producing transcripts capable of assuming a double-stranded RNA structure.
(Japanese National Institute of Genetics, 2012.5.21)
Cell lines
Observed in
Not observed in
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hide Stocks Listed in FlyBase ( 0 )
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hide Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym
11315R
(Japanese National Institute of Genetics, 2012.5.21)
dsRNA-11315R
 
Secondary FlyBase IDs
    hide References ( 1 )
    Stock list
    Japanese National Institute of Genetics, 2012.5.21, RNAi stocks available from NIG-FLY.
    RNAi stocks available from NIG-FLY. [FBrf0218457]