A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Sequence Feature: RNAi_reagent

General Information
Symbol dsRNA-11676R Species D. melanogaster
Feature type RNAi_reagent FlyBase ID FBsf0000429141
Collection NIG_RNAi_Fly-1   Associated gene(s) Glut4EF
Genomic Location
Chromosome (arm) 3R Sequence location 3R:5,812,630..5,813,129 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBtr0333686 FBtr0333685 FBtr0112576 FBtr0305157 FBtr0112577 FBtr0082150 FBtr0333687 FBsf0000015266 FBsf0000028663 FBsf0000026187 FBsf0000021466 FBsf0000013841 FBsf0000035776 FBsf0000035775 FBsf0000087289 FBsf0000074936 FBsf0000091822 FBsf0000076723 FBsf0000079477 FBsf0000151677 FBsf0000428240 FBsf0000400285 FBsf0000407100 FBsf0000394872 FBsf0000393536
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Description
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This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
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FB2013_03
FB2013_02
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hide Sequence Data
Length 507
Comments
One copy of repeat shown.
(Japanese National Institute of Genetics, 2012.5.21)
Sequence 
gCGGTAGCCACTACTAACCATCTAGCGCAGTCGCACGGACGCACCATCAG
TGGCAGCAACATCCCGAGCACCCACCAGCAGCAGCTGCAGAAACAACAAC
ACCAGCTGCATCCCCACTTCGCACCTAGCCCACCACAACTACACATGCCC
GGCGGCGACGGCGACAGTGGGCAGCTACTCCTCCACGGGGACGGGGTCGG
TGGCGGCCAGTTCATCAGCCTCGCCGATCGGCAAGCACGCTCGCTCGTCG
TCCTCCCGTCCGACCCACTCGGTAGCGCCATATCCATCGCCGACGTACGT
CCAGCAACAGCAGCACCACCAACACACGCACCACCACAATTACGCCGGCA
GCAGCGGCAGCAGCAAACAGCAGCAGCAGCAGCAGTCCAGTGATCCACAG
CAATAGCAGTGCCAACAACATGCTGCGGCAGNNTGTCGCAGCAGAAACGT
CACAGTGGACGGNCGCACCACAGCCAGCAGCAGCAGCAACTgcaGCAGCA
GCAACAC
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Gene(s)
(targeted or local)
Allele(s)
Transcripts(s)
Polypeptide(s)
Construct
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PCR template
cDNA
(Japanese National Institute of Genetics, 2012.5.21)
Primer 1
aaggcctacatggccggaccgGCGGTAGCCACTATAACCATC
(Japanese National Institute of Genetics, 2012.5.21)
Primer 2
aatctagaggtaccGTGTTGCTGCTGCTGCAGTTG
(Japanese National Institute of Genetics, 2012.5.21)
Comments
hide Collection Information
hide Collection: NIG_RNAi_Fly-1
Symbol NIG_RNAi_Fly-1
Source and Progenitors
Species of derivation D. melanogaster  
Strain of derivation
Vector or progenitor construct
Description
Comprehensive collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene.
(Japanese National Institute of Genetics, 2012.5.21)
Additional data
A collection of over 6,400 RNAi transgenes (over 12,300 lines), each designed to target a single protein-coding gene. Each construct contains a pair of identical 500-bp amplicons produced using cDNA templates. These have been cloned as inverted repeats that are expressed using the binary GAL4/UAS system, producing transcripts capable of assuming a double-stranded RNA structure.
(Japanese National Institute of Genetics, 2012.5.21)
Cell lines
Observed in
Not observed in
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hide Stocks Listed in FlyBase ( 0 )
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hide Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym
11676R
(Japanese National Institute of Genetics, 2012.5.21)
dsRNA-11676R
 
Secondary FlyBase IDs
    hide References ( 1 )
    Stock list
    Japanese National Institute of Genetics, 2012.5.21, RNAi stocks available from NIG-FLY.
    RNAi stocks available from NIG-FLY. [FBrf0218457]