A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Sequence Feature: RNAi_reagent

General Information
Symbol dsRNA-11860R Species D. melanogaster
Feature type RNAi_reagent FlyBase ID FBsf0000429204
Collection NIG_RNAi_Fly-1   Associated gene(s) yuri
Genomic Location
Chromosome (arm) 2L Sequence location 2L:15,263,511..15,264,115 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBtr0336862 FBtr0336863 FBtr0300932 FBtr0080709 FBtr0080712 FBtr0080711 FBtr0336861 FBtr0302159 FBtr0302158 FBtr0302156 FBtr0302157 FBsf0000001851 FBsf0000001878 FBsf0000026470 FBsf0000094058 FBsf0000413738 FBsf0000410245 FBsf0000385242 FBsf0000385269
hide Recent Updates
Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
Update Feed
Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
atom feed
FB2013_03
FB2013_02
All updates Click here to see a list of all updates to this record from FB2010_08 and on.
hide Sequence Data
Length 501
Comments
One copy of repeat shown.
(Japanese National Institute of Genetics, 2012.5.21)
Sequence 
CCAAGCCGGTTTCGTCCTAGACGCCAAGTCCATGGGTCTGAATCCGTCGA
GACCTGCATCATCGCCACCGCCACCACCATCATCTGCCAGTCAAATGTGT
CGGCAAGTGCAGCCANACCGGAACGAGTGCCTCGCATCCACATCAAGAAT
CGACGCTGAAAGCCTCATCTCCTGATCGGTGCACTGCCAGCACCGAGGCG
ATGTCCNCCGAACATTACCGCTGTCTGATCCAGAAGCTGCGGGATACCAA
CGGCGATGCTGGCGATATGAGCTTTGAGCTGAACAGCATATTTCTGAAGC
GGCTGGACGAGATTGACTGCCTAGATGAGCACGAGGGTGATGACATGCAC
ACTTGCCAGTTGCGTCTGGTCACTTTTCAGGAGTGGGTGGATTTCCTGCT
CCACGTGAACAGCGTGATCCTGGGCAACGTGTCGGACTTGGAGAAGGAGG
CGTACAGCAAGATCGTTGCCTGCTGTCAGTCTGTCCAAGTGCGACAACAG
C
hide Associated Information
Gene(s)
(targeted or local)
Allele(s)
Transcripts(s)
Polypeptide(s)
Construct
hide Experimental Data
hide Progenitors
PCR template
cDNA
(Japanese National Institute of Genetics, 2012.5.21)
Primer 1
aaggcctacatggccggaccgCCAAGCCGGTTTCGTCCTAGA
(Japanese National Institute of Genetics, 2012.5.21)
Primer 2
aatctagaggtaccGCTGTTGTCGCACTTGGACAG
(Japanese National Institute of Genetics, 2012.5.21)
Comments
hide Collection Information
hide Collection: NIG_RNAi_Fly-1
Symbol NIG_RNAi_Fly-1
Source and Progenitors
Species of derivation D. melanogaster  
Strain of derivation
Vector or progenitor construct
Description
Comprehensive collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene.
(Japanese National Institute of Genetics, 2012.5.21)
Additional data
A collection of over 6,400 RNAi transgenes (over 12,300 lines), each designed to target a single protein-coding gene. Each construct contains a pair of identical 500-bp amplicons produced using cDNA templates. These have been cloned as inverted repeats that are expressed using the binary GAL4/UAS system, producing transcripts capable of assuming a double-stranded RNA structure.
(Japanese National Institute of Genetics, 2012.5.21)
Cell lines
Observed in
Not observed in
hide Comments
hide Stocks Listed in FlyBase ( 0 )
hide External Crossreferences & Linkouts
hide Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym
11860R
(Japanese National Institute of Genetics, 2012.5.21)
dsRNA-11860R
 
Secondary FlyBase IDs
    hide References ( 1 )
    Stock list
    Japanese National Institute of Genetics, 2012.5.21, RNAi stocks available from NIG-FLY.
    RNAi stocks available from NIG-FLY. [FBrf0218457]