A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Sequence Feature: RNAi_reagent

General Information
Symbol dsRNA-1211R Species D. melanogaster
Feature type RNAi_reagent FlyBase ID FBsf0000429330
Collection NIG_RNAi_Fly-1   Associated gene(s) CG33965
Genomic Location
Chromosome (arm) 3L Sequence location 3L:1,232,957..1,233,509 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBtr0331393 FBtr0336737 FBtr0331392 FBtr0100006 FBtr0100007 FBsf0000008062 FBsf0000025014 FBsf0000033910 FBsf0000007937 FBsf0000023317 FBsf0000077379 FBsf0000088615 FBsf0000076833 FBsf0000402789 FBsf0000412024 FBsf0000403434 FBsf0000390048 FBsf0000389933
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
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FB2013_03
FB2013_02
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hide Sequence Data
Length 500
Comments
One copy of repeat shown.
(Japanese National Institute of Genetics, 2012.5.21)
Sequence 
AGTGGCCATCGAAGAGCTGAACGAAGTGCCAAGTCGTGTGGAATCGGACA
TTGCTGCTCTGAAGGAATGGCTACAGAAGCAGCCCCATCTCTGCGCCTGT
CTCGAGGATCAGTTCCTGCTCAGTTTTCTTAGGGGATCGAAGTTTAGTCT
GGAGAAGGCCAAGCAGAAGATTGATCGATTCTATAGCCTGCAAGCGGTCA
TCCCAGAGGTTTTCAACGATCAGCGATTGGTTGACAATGCCCAGGTGTTG
GAAATTATTCGACTGGGAGTGATCTTGCGCATTCCCCTCGATGAGGAGGA
TACTGGACCAGCCGTGACTATTATTCGAGCTGGCTCCTACGACATCAATA
AATTCAAGTTCCAGGACATCATACGTGTGGGCTCCATGTTCGGTGAGATC
ATGATGCTGGAGGATGACAATGCCTCCGTCAGTGGCTACCTGGAGATCAT
GGACATGAGTGGAGTGACGGGTGCAAATCTGTTTGCCCTCCAACCCCAAC
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Gene(s)
(targeted or local)
Allele(s)
Transcripts(s)
Polypeptide(s)
Construct
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PCR template
cDNA
(Japanese National Institute of Genetics, 2012.5.21)
Primer 1
aaggcctacatggccggaccgAGTGGCCATCGAAGAGCTGAA
(Japanese National Institute of Genetics, 2012.5.21)
Primer 2
aatctagaggtaccGTTGGGGTTGGAGGGCAAACA
(Japanese National Institute of Genetics, 2012.5.21)
Comments
hide Collection Information
hide Collection: NIG_RNAi_Fly-1
Symbol NIG_RNAi_Fly-1
Source and Progenitors
Species of derivation D. melanogaster  
Strain of derivation
Vector or progenitor construct
Description
Comprehensive collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene.
(Japanese National Institute of Genetics, 2012.5.21)
Additional data
A collection of over 6,400 RNAi transgenes (over 12,300 lines), each designed to target a single protein-coding gene. Each construct contains a pair of identical 500-bp amplicons produced using cDNA templates. These have been cloned as inverted repeats that are expressed using the binary GAL4/UAS system, producing transcripts capable of assuming a double-stranded RNA structure.
(Japanese National Institute of Genetics, 2012.5.21)
Cell lines
Observed in
Not observed in
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hide Stocks Listed in FlyBase ( 0 )
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hide Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym
1211R
(Japanese National Institute of Genetics, 2012.5.21)
dsRNA-1211R
 
Secondary FlyBase IDs
    hide References ( 1 )
    Stock list
    Japanese National Institute of Genetics, 2012.5.21, RNAi stocks available from NIG-FLY.
    RNAi stocks available from NIG-FLY. [FBrf0218457]