A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Sequence Feature: RNAi_reagent

General Information
Symbol dsRNA-18444R Species D. melanogaster
Feature type RNAi_reagent FlyBase ID FBsf0000431129
Collection NIG_RNAi_Fly-1   Associated gene(s) αTry
Genomic Location
Chromosome (arm) 2R Sequence location 2R:7,232,157..7,232,589 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBtr0088162 FBtr0088161 FBtr0088160 FBtr0088122 FBsf0000027382 FBsf0000007318 FBsf0000022717 FBsf0000007352 FBsf0000020937 FBsf0000007323 FBsf0000027490 FBsf0000094457 FBsf0000075199 FBsf0000083182 FBsf0000094744 FBsf0000410715 FBsf0000411137 FBsf0000411123 FBsf0000389579 FBsf0000389612 FBsf0000389584 FBsf0000431129
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
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This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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hide Sequence Data
Length 433
Comments
One copy of repeat shown.
(Japanese National Institute of Genetics, 2012.5.21)
Sequence 
GATCGTGATCCTCTTGTCCGCCGTGGTCTGCGCCCTGGGAGGCACCGTCC
CTGAGGGACTCCTGCCTCAGTTGGATGGCCGCATTGTCGGCGGCTCTGCT
ACCACCATCAGCAGCTTCCCCTGGCAGATCTCCCTGCAGCGCAGTGGCAG
CCACTCCTGCGGTGGATCCATCTACTCTGCCAACATCATTGTGACCGCCG
CTCACTGTCTGCAGTCCGTGTCCGCTTCAGTCCTGCAGGTCCGTGCTGGA
TCCACCTACTGGAGCTCTGGTGGCGTCGTCGCCAAGGTTTCCTCTTTCAA
GAACCACGAGGGATACAACGCTAACACCATGGTCAACGACATCGCTGTCA
TCCGTCTGAGCTCTTCCCTGAGCTTCAGCTCAAGCATCAAGGCTATTAGC
CTGGCCACTTACAACCCAGCTAACGGAGCCTCT
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Gene(s)
(targeted or local)
Allele(s)
Transcripts(s)
Polypeptide(s)
Construct
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PCR template
cDNA
(Japanese National Institute of Genetics, 2012.5.21)
Primer 1
aaggcctacatggccggaccgGATCGTGATCCTCTTGTCCGC
(Japanese National Institute of Genetics, 2012.5.21)
Primer 2
aatctagaggtaccAGAGGCTCCGTTAGCTGGGTT
(Japanese National Institute of Genetics, 2012.5.21)
Comments
hide Collection Information
hide Collection: NIG_RNAi_Fly-1
Symbol NIG_RNAi_Fly-1
Source and Progenitors
Species of derivation D. melanogaster  
Strain of derivation
Vector or progenitor construct
Description
Comprehensive collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene.
(Japanese National Institute of Genetics, 2012.5.21)
Additional data
A collection of over 6,400 RNAi transgenes (over 12,300 lines), each designed to target a single protein-coding gene. Each construct contains a pair of identical 500-bp amplicons produced using cDNA templates. These have been cloned as inverted repeats that are expressed using the binary GAL4/UAS system, producing transcripts capable of assuming a double-stranded RNA structure.
(Japanese National Institute of Genetics, 2012.5.21)
Cell lines
Observed in
Not observed in
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hide Stocks Listed in FlyBase ( 0 )
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hide Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym
18444R
(Japanese National Institute of Genetics, 2012.5.21)
dsRNA-18444R
 
Secondary FlyBase IDs
    hide References ( 1 )
    Stock list
    Japanese National Institute of Genetics, 2012.5.21, RNAi stocks available from NIG-FLY.
    RNAi stocks available from NIG-FLY. [FBrf0218457]