A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Sequence Feature: RNAi_reagent

General Information
Symbol dsRNA-31015R Species D. melanogaster
Feature type RNAi_reagent FlyBase ID FBsf0000431643
Collection NIG_RNAi_Fly-1   Associated gene(s) PH4αPV
Genomic Location
Chromosome (arm) 3R Sequence location 3R:26,473,473..26,474,124 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBtr0085671 FBtr0085670 FBtr0112733 FBtr0112734 FBtr0304711 FBsf0000032501 FBsf0000020957 FBsf0000025589 FBsf0000034795 FBsf0000014570 FBsf0000090467 FBsf0000075928 FBsf0000088627 FBsf0000071383 FBsf0000405183 FBsf0000403448 FBsf0000411766 FBsf0000394210
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Description
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This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
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FB2013_03
FB2013_02
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hide Sequence Data
Length 502
Comments
One copy of repeat shown.
(Japanese National Institute of Genetics, 2012.5.21)
Sequence 
ACTGCACCTCGGTGGCAGGAATGGTCAAGCTGCTCGATTTGGAGGCGCAN
GCTGATCGACAACCTAGAGGATTACGCTGTCGAGCTGGAGAAGAAACTGC
AGACCGTAAGAAGGAGCATAACAAGTCTGCGTTTGGAAAATGACAAAGCC
CGCAGTTCCACGGAGGAGTACTTATCCAATCCCCTGAACTCCTTTTCCCT
CATTCGTCGCATGAATCGCGACTGGATTATTTGGCAACTCTACATGGACG
ATCCGGTGGGTATTTCTCAGGTGGAGCGAATCCAGGAGTTGAGGGAACAC
ATGCCCACCCACACGGATGTGGAAGGAGGCTGTCACTGCCTTGGATCGCA
TTCAAAGCACCTATGGCCTTAAAGTGCCGGAAATCTCCCATGGATTCCTC
AATGGCAAACAGTACAATGTGAGTCTCACTGTATTGGATACCTATGCCAT
GGGACAAATCTTATTTGACCAGAAAAACTATTTGGCAGCTGCTAGCTGGA
TC
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Gene(s)
(targeted or local)
Allele(s)
Transcripts(s)
Polypeptide(s)
Construct
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PCR template
cDNA
(Japanese National Institute of Genetics, 2012.5.21)
Primer 1
aaggcctacatggccggaccgACTGCACCTCGGTGGCAGGAA
(Japanese National Institute of Genetics, 2012.5.21)
Primer 2
aatctagaggtaccGATCCAGCTAGCAGCTGCCAA
(Japanese National Institute of Genetics, 2012.5.21)
Comments
hide Collection Information
hide Collection: NIG_RNAi_Fly-1
Symbol NIG_RNAi_Fly-1
Source and Progenitors
Species of derivation D. melanogaster  
Strain of derivation
Vector or progenitor construct
Description
Comprehensive collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene.
(Japanese National Institute of Genetics, 2012.5.21)
Additional data
A collection of over 6,400 RNAi transgenes (over 12,300 lines), each designed to target a single protein-coding gene. Each construct contains a pair of identical 500-bp amplicons produced using cDNA templates. These have been cloned as inverted repeats that are expressed using the binary GAL4/UAS system, producing transcripts capable of assuming a double-stranded RNA structure.
(Japanese National Institute of Genetics, 2012.5.21)
Cell lines
Observed in
Not observed in
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hide Stocks Listed in FlyBase ( 0 )
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hide Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym
31015R
(Japanese National Institute of Genetics, 2012.5.21)
dsRNA-31015R
 
Secondary FlyBase IDs
    hide References ( 1 )
    Stock list
    Japanese National Institute of Genetics, 2012.5.21, RNAi stocks available from NIG-FLY.
    RNAi stocks available from NIG-FLY. [FBrf0218457]