A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Sequence Feature: RNAi_reagent

General Information
Symbol dsRNA-31463R Species D. melanogaster
Feature type RNAi_reagent FlyBase ID FBsf0000431746
Collection NIG_RNAi_Fly-1   Associated gene(s) CG31463
Genomic Location
Chromosome (arm) 3R Sequence location 3R:4,118,095..4,118,594 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBtr0332542 FBtr0081865 FBtr0306804 FBtr0081831 FBtr0081864 FBtr0332541 FBtr0306806 FBtr0306805 FBsf0000030432 FBsf0000031698 FBsf0000013839 FBsf0000031699 FBsf0000031697 FBsf0000013840 FBsf0000024140 FBsf0000035504 FBsf0000077862 FBsf0000080814 FBsf0000401755 FBsf0000402233 FBsf0000401799 FBsf0000393534 FBsf0000393535 FBsf0000431746
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
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This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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hide Sequence Data
Length 500
Comments
One copy of repeat shown.
(Japanese National Institute of Genetics, 2012.5.21)
Sequence 
GAAAAGCATTGCTTCTGCTGGGCTGTGTGCTGCTCTTCGCTTTTGGTGAA
GCCAACCAGGAACTGGAGACTCTGCTGCAGAAGCAACAGGTGGACTTGCA
GTTGTCTGGCGAGGAAGTGGCTGCTTTGCGTCCACTGTACCTAGAGTTTC
AGGCGCAGTACAACTATCTGTACAGCCTGAGGCTCCAGAGCGGCGATGGG
AGTGATCAGAATCCCGCGGAGGAGCCTGAACTGGATAGCAACTACCTACG
GGCATTTGAAGACTTTCGCGAAAAGTTCTCCATTTACTTGGATGAGAAAC
CTCGAGTGGTGTATGACACAAAATTGCCCACTGTCGACGAGATCATGGGA
AAGCCCAGTCCCGACTTCACACCAAATCAGAGGGCGGAATTCGAGGATCT
CAGGGAGATTATTCAGGATGTTATTGACGAGGCACAGTTCGGCATTGACG
ACTTGGTGGAGAGGGCCATCCAACTGGAGACGAATCTGCTGAAGCTGAAC
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Gene(s)
(targeted or local)
Allele(s)
Transcripts(s)
Polypeptide(s)
Construct
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PCR template
cDNA
(Japanese National Institute of Genetics, 2012.5.21)
Primer 1
aaggcctacatggccggaccgGAAAAGCATTGCTTCTGCTGG
(Japanese National Institute of Genetics, 2012.5.21)
Primer 2
aatctagaggtaccGTTCAGCTTCAGCAGATTCGT
(Japanese National Institute of Genetics, 2012.5.21)
Comments
hide Collection Information
hide Collection: NIG_RNAi_Fly-1
Symbol NIG_RNAi_Fly-1
Source and Progenitors
Species of derivation D. melanogaster  
Strain of derivation
Vector or progenitor construct
Description
Comprehensive collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene.
(Japanese National Institute of Genetics, 2012.5.21)
Additional data
A collection of over 6,400 RNAi transgenes (over 12,300 lines), each designed to target a single protein-coding gene. Each construct contains a pair of identical 500-bp amplicons produced using cDNA templates. These have been cloned as inverted repeats that are expressed using the binary GAL4/UAS system, producing transcripts capable of assuming a double-stranded RNA structure.
(Japanese National Institute of Genetics, 2012.5.21)
Cell lines
Observed in
Not observed in
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hide Stocks Listed in FlyBase ( 0 )
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hide Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym
31463R
(Japanese National Institute of Genetics, 2012.5.21)
dsRNA-31463R
 
Secondary FlyBase IDs
    hide References ( 1 )
    Stock list
    Japanese National Institute of Genetics, 2012.5.21, RNAi stocks available from NIG-FLY.
    RNAi stocks available from NIG-FLY. [FBrf0218457]