A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Sequence Feature: RNAi_reagent

General Information
Symbol dsRNA-4861R Species D. melanogaster
Feature type RNAi_reagent FlyBase ID FBsf0000432711
Collection NIG_RNAi_Fly-1   Associated gene(s) LpR1
Genomic Location
Chromosome (arm) 3R Sequence location 3R:21,571,883..21,573,865 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBtr0113480 FBtr0301466 FBtr0335238 FBtr0301470 FBtr0301467 FBtr0301471 FBtr0335235 FBtr0301469 FBtr0335237 FBtr0335236 FBsf0000025419 FBsf0000015154 FBsf0000092724 FBsf0000073249 FBsf0000071044 FBsf0000095443 FBsf0000408394 FBsf0000394765
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Description
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This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
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FB2013_03
FB2013_02
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hide Sequence Data
Length 501
Comments
One copy of repeat shown.
(Japanese National Institute of Genetics, 2012.5.21)
Sequence 
GAGCAAGGACTGCAGCGATGGCTCCGACGAGCACAACTGCAACCAGACCT
GTCGTGCCGACGAGTTCACCTGCGGCAATGGACGGTGCATCCAGAAGCGA
TGGAAGTGCGACCACGACGACGACTGCGGCGATGGATCCGACGAGAAGGA
GTGCCCAGTGGTGCCCTGCGACTCCGTGGCGGAGCACACCTGCACGAATG
GAGCCTGCATCGCCAAGCGGTGGGTCTGCGACGGTGATCCGGACTGCTCC
GATGGCTCCGATGAGCGGTCCTGTGCGAATGTGACCAAGACGACCACGCC
CTGTTTATCGCACGAATACCAGTGCAAGGATCGCATCACCTGCCTGCATC
ACAGCTGGNTCTGCGATGGTGACCGCGACTGTCCGGATGGCGACGATGAG
CACACGGCCAACTGCAAGAACGTCACCTGCCGGGCGGACCAGTTCCAGCT
GCGGCGATCGCAGCTGCATTCCAGGACATCTCACCTGCAACGGCGACAAG
G
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Gene(s)
(targeted or local)
Allele(s)
Transcripts(s)
Polypeptide(s)
Construct
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PCR template
cDNA
(Japanese National Institute of Genetics, 2012.5.21)
Primer 1
aaggcctacatggccggaccgGAGCAAGGACTGCAGCGATGG
(Japanese National Institute of Genetics, 2012.5.21)
Primer 2
aatctagaggtaccCCTTGTCGCCGTTGCAGGTGA
(Japanese National Institute of Genetics, 2012.5.21)
Comments
hide Collection Information
hide Collection: NIG_RNAi_Fly-1
Symbol NIG_RNAi_Fly-1
Source and Progenitors
Species of derivation D. melanogaster  
Strain of derivation
Vector or progenitor construct
Description
Comprehensive collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene.
(Japanese National Institute of Genetics, 2012.5.21)
Additional data
A collection of over 6,400 RNAi transgenes (over 12,300 lines), each designed to target a single protein-coding gene. Each construct contains a pair of identical 500-bp amplicons produced using cDNA templates. These have been cloned as inverted repeats that are expressed using the binary GAL4/UAS system, producing transcripts capable of assuming a double-stranded RNA structure.
(Japanese National Institute of Genetics, 2012.5.21)
Cell lines
Observed in
Not observed in
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hide Stocks Listed in FlyBase ( 0 )
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hide Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym
4861R
(Japanese National Institute of Genetics, 2012.5.21)
dsRNA-4861R
 
Secondary FlyBase IDs
    hide References ( 1 )
    Stock list
    Japanese National Institute of Genetics, 2012.5.21, RNAi stocks available from NIG-FLY.
    RNAi stocks available from NIG-FLY. [FBrf0218457]