A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Sequence Feature: RNAi_reagent

General Information
Symbol dsRNA-6121R Species D. melanogaster
Feature type RNAi_reagent FlyBase ID FBsf0000433305
Collection NIG_RNAi_Fly-1   Associated gene(s) Tip60
Genomic Location
Chromosome (arm) X Sequence location X:4,010,169..4,010,722 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBtr0333184 FBtr0333183 FBtr0070642 FBtr0333186 FBtr0305182 FBtr0305180 FBtr0305181 FBtr0300931 FBtr0070643 FBtr0333185 FBtr0070668 FBsf0000018078 FBsf0000026136 FBsf0000033808 FBsf0000018080 FBsf0000028377 FBsf0000028376 FBsf0000079952 FBsf0000089171 FBsf0000195040 FBsf0000080292 FBsf0000095569 FBsf0000382524 FBsf0000402551 FBsf0000405966 FBsf0000397207 FBsf0000397209
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Description
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This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
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FB2013_03
FB2013_02
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hide Sequence Data
Length 500
Comments
One copy of repeat shown.
(Japanese National Institute of Genetics, 2012.5.21)
Sequence 
ATGCACAAAACGGACGACTGGCCACTANCGGAGATTGTGAGCATCAAGGA
GTTGGATGGGCGCCGGCAGTTTTACGTGCACTATGTGGACTTCAACAAGC
GCTTGGACGAGTGGGTCAACGAGGAGGATCTGTACACGCGAAAGGTGCAA
TTTCCGCGGAGAGACGGCTCACAAACAGGCACCAGCACTGGAGTGACCAC
GCCACAGCGCCACCATTCCCTAGCGGGCAGCNTTTCTCGGCCTACATCAC
CGCAACACCCTGGTTCCGGGGCTCTGGCAGCCATCCCCCAGACTCCTACT
GGTGCATCAGGCAGTGTTCCTCCCCCTGCTGGCATTCCAAACTCTGTGGC
TCCACCCGGAACACCAAGCAGTGGGGGAGAGCTTGTGAATGGCAACAATC
TGGCCGCAGCCCTTCAGAAACGCATCAATCGCAAGCGAAAAAACCACGGA
GGCTCGGCCCATGGACACCACAGTCTGACCAGTCAACAGCAGCAGTCACA
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Gene(s)
(targeted or local)
Allele(s)
Transcripts(s)
Polypeptide(s)
Construct
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PCR template
cDNA
(Japanese National Institute of Genetics, 2012.5.21)
Primer 1
aaggcctacatggccggaccgATGCACAAAACGGACGACTGG
(Japanese National Institute of Genetics, 2012.5.21)
Primer 2
aatctagaggtaccTGTGACTGCTGCTGTTGACTG
(Japanese National Institute of Genetics, 2012.5.21)
Comments
hide Collection Information
hide Collection: NIG_RNAi_Fly-1
Symbol NIG_RNAi_Fly-1
Source and Progenitors
Species of derivation D. melanogaster  
Strain of derivation
Vector or progenitor construct
Description
Comprehensive collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene.
(Japanese National Institute of Genetics, 2012.5.21)
Additional data
A collection of over 6,400 RNAi transgenes (over 12,300 lines), each designed to target a single protein-coding gene. Each construct contains a pair of identical 500-bp amplicons produced using cDNA templates. These have been cloned as inverted repeats that are expressed using the binary GAL4/UAS system, producing transcripts capable of assuming a double-stranded RNA structure.
(Japanese National Institute of Genetics, 2012.5.21)
Cell lines
Observed in
Not observed in
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hide Stocks Listed in FlyBase ( 0 )
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hide Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym
6121R
(Japanese National Institute of Genetics, 2012.5.21)
dsRNA-6121R
 
Secondary FlyBase IDs
    hide References ( 1 )
    Stock list
    Japanese National Institute of Genetics, 2012.5.21, RNAi stocks available from NIG-FLY.
    RNAi stocks available from NIG-FLY. [FBrf0218457]