A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Sequence Feature: RNAi_reagent

General Information
Symbol dsRNA-6645R Species D. melanogaster
Feature type RNAi_reagent FlyBase ID FBsf0000433555
Collection NIG_RNAi_Fly-1   Associated gene(s) CG32054
Genomic Location
Chromosome (arm) 3L Sequence location 3L:10,086,920..10,087,615 [-]
Map ( GBrowse ) GBrowse View Help detailed view FBtr0332666 FBtr0332665 FBtr0076315 FBtr0076314 FBtr0303833 FBsf0000022263 FBsf0000010310 FBsf0000031748 FBsf0000022393 FBsf0000010307 FBsf0000088635 FBsf0000073039 FBsf0000088349 FBsf0000073040 FBsf0000403456 FBsf0000402307 FBsf0000391544 FBsf0000391541
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
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FB2013_03
FB2013_02
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hide Sequence Data
Length 501
Comments
One copy of repeat shown.
(Japanese National Institute of Genetics, 2012.5.21)
Sequence 
TACCCACCGCCACCTCAACAAGGATATCCCCCACAGTTTTACGGTCCACC
ACCAGGATACGGATTACCACAAGCAACACTACCTCCTAATCCGCCCCAGC
AAGCCATCACGGTAAATAACAGCTGGTACAGTCGTAATCAGAAGAACAAG
CCACAATCGAATGCTGTTGGAGCAGCGGGTCTCATTTTCGTCTCTGGCGG
TATGAATATTGCGTGGAGCATTGGATTCAGAGGAGTACTCTACTATAAAA
CCACTGAGCATAATTTTGCAGCGTGGTTCATTGGTGGCATTATAGGAGCT
GTGATCAGTTGGTTTCTCATAAACAAAGTGGCCAAGAAGTATGTCTTGAT
CTTCAGTTCGTTTCTGGTGATGATTGGCGGAATACTGACTACCAGTACAA
AGAATATGTGGCGNTGCCACGTTGGCGGCTTCCTATCTGGATGGAATCGC
CAATGGACTGGTGTTTGCTCCATTCATgGCCTTGGCCGGAGAGGTCTCAG
T
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Gene(s)
(targeted or local)
Allele(s)
Transcripts(s)
Polypeptide(s)
Construct
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PCR template
cDNA
(Japanese National Institute of Genetics, 2012.5.21)
Primer 1
aaggcctacatggccggaccgTACCCACCGCCACCTCAACAA
(Japanese National Institute of Genetics, 2012.5.21)
Primer 2
aatctagaggtaccACTGAGACCTCTCCGGCCAAG
(Japanese National Institute of Genetics, 2012.5.21)
Comments
hide Collection Information
hide Collection: NIG_RNAi_Fly-1
Symbol NIG_RNAi_Fly-1
Source and Progenitors
Species of derivation D. melanogaster  
Strain of derivation
Vector or progenitor construct
Description
Comprehensive collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene.
(Japanese National Institute of Genetics, 2012.5.21)
Additional data
A collection of over 6,400 RNAi transgenes (over 12,300 lines), each designed to target a single protein-coding gene. Each construct contains a pair of identical 500-bp amplicons produced using cDNA templates. These have been cloned as inverted repeats that are expressed using the binary GAL4/UAS system, producing transcripts capable of assuming a double-stranded RNA structure.
(Japanese National Institute of Genetics, 2012.5.21)
Cell lines
Observed in
Not observed in
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hide Stocks Listed in FlyBase ( 0 )
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hide Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym
6645R
(Japanese National Institute of Genetics, 2012.5.21)
dsRNA-6645R
 
Secondary FlyBase IDs
    hide References ( 1 )
    Stock list
    Japanese National Institute of Genetics, 2012.5.21, RNAi stocks available from NIG-FLY.
    RNAi stocks available from NIG-FLY. [FBrf0218457]