A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Sequence Feature: RNAi_reagent

General Information
Symbol dsRNA-6990R Species D. melanogaster
Feature type RNAi_reagent FlyBase ID FBsf0000433703
Collection NIG_RNAi_Fly-1   Associated gene(s) HP1c
Genomic Location
Chromosome (arm) 3R Sequence location 3R:18,571,449..18,571,948 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBtr0084323 FBtr0084309 FBtr0084322 FBtr0339522 FBtr0084321 FBsf0000015129 FBsf0000025989 FBsf0000015651 FBsf0000014671 FBsf0000020255 FBsf0000088414 FBsf0000079926 FBsf0000094144 FBsf0000083084 FBsf0000080533 FBsf0000078228 FBsf0000082818 FBsf0000075816 FBsf0000151667 FBsf0000169263 FBsf0000402406 FBsf0000410350 FBsf0000406028 FBsf0000413732 FBsf0000394740 FBsf0000395251 FBsf0000394307 FBsf0000433703
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Description
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This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
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FB2013_03
FB2013_02
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hide Sequence Data
Length 500
Comments
One copy of repeat shown.
(Japanese National Institute of Genetics, 2012.5.21)
Sequence 
ACGAGCCCAACTTCGTGGTGGAGCGCATCATGGACAAGCGCATTACCAGC
GAAGGCAAGGTTGAGTACTACATCAAGTGGCGTGGCTACACGTCGGCGGA
CAACACCTGGGAGCCCGAGGAGAACTGCGATTGCCCGAATCTCATCCAGA
AATTCGAGGAGTCGCGCGCCAAGTCCAAGAAGCGCGGCGAGAAGAAACCC
AAGTGCGAAGAGATCCAGAAGCTGCGCGGCTACGAGCGCGGCTTGGAGCT
GGCCGAGATCGTGGGCGCAACGGATGTGACGGGCGACATCAAGTATCTGG
TACGCTGGCAGTTCTGCGACGAGTTCGACTTGGTGCCATCGGCACAGATC
GTGGAGAAGGATCCGCAAATGCTGATTGACTATTTCCAGAAGATGGCACC
TTACTCCCGTCACATTGCGATGCGAATGAAGGGCGTGCCGGAGGAGCTGC
GTTTGGCGGCCTCGCGCACCAGTTATCCGCACATTAGCAGTGCGCCCGTC
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Gene(s)
(targeted or local)
Allele(s)
Transcripts(s)
Polypeptide(s)
Construct
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PCR template
cDNA
(Japanese National Institute of Genetics, 2012.5.21)
Primer 1
aaggcctacatggccggaccgACGAGCCCAACTTCGTGGTGG
(Japanese National Institute of Genetics, 2012.5.21)
Primer 2
aatctagaggtaccGACGGGCGCACTGCTAATGTG
(Japanese National Institute of Genetics, 2012.5.21)
Comments
hide Collection Information
hide Collection: NIG_RNAi_Fly-1
Symbol NIG_RNAi_Fly-1
Source and Progenitors
Species of derivation D. melanogaster  
Strain of derivation
Vector or progenitor construct
Description
Comprehensive collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene.
(Japanese National Institute of Genetics, 2012.5.21)
Additional data
A collection of over 6,400 RNAi transgenes (over 12,300 lines), each designed to target a single protein-coding gene. Each construct contains a pair of identical 500-bp amplicons produced using cDNA templates. These have been cloned as inverted repeats that are expressed using the binary GAL4/UAS system, producing transcripts capable of assuming a double-stranded RNA structure.
(Japanese National Institute of Genetics, 2012.5.21)
Cell lines
Observed in
Not observed in
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hide Stocks Listed in FlyBase ( 0 )
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hide Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym
6990R
(Japanese National Institute of Genetics, 2012.5.21)
dsRNA-6990R
 
Secondary FlyBase IDs
    hide References ( 1 )
    Stock list
    Japanese National Institute of Genetics, 2012.5.21, RNAi stocks available from NIG-FLY.
    RNAi stocks available from NIG-FLY. [FBrf0218457]