A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Sequence Feature: RNAi_reagent

General Information
Symbol dsRNA-7719R Species D. melanogaster
Feature type RNAi_reagent FlyBase ID FBsf0000434031
Collection NIG_RNAi_Fly-1   Associated gene(s) gwl
Genomic Location
Chromosome (arm) 3R Sequence location 3R:14,572,323..14,573,337 [+]
Map ( GBrowse ) GBrowse View Help detailed view FBtr0083682 FBtr0331352 FBtr0083674 FBsf0000015789 FBsf0000035874 FBsf0000015790 FBsf0000035875 FBsf0000194238 FBsf0000078772 FBsf0000078773 FBsf0000382490 FBsf0000169185 FBsf0000399168 FBsf0000414994 FBsf0000399486 FBsf0000414396 FBsf0000395383 FBsf0000395384
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Description
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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hide Sequence Data
Length 500
Comments
One copy of repeat shown.
(Japanese National Institute of Genetics, 2012.5.21)
Sequence 
GGATGTCCACATCGACTACAAAACCCCCAAGAAGACCCACTCGCTGATCG
ACAGTGAGCAGTTGCTGGACAAGATCAACATCCTTACCACCAAGCCGGAG
AATCACTCCCAGAATGCCAAGCTTCCCACTATCAAGGATTTTGTCATCAT
TAAGCCAATCAGCCGCGGCGCCTTTGGAAAAGTCTTCCTGGGCTACAAGA
ACAACGACTCGAAGCGGCTGTTCGCCATCAAGGTGATGCGCAAGTCAGAG
ATGATCAACAAGAACATGGTGTCCCAGGTAATCACGGAGCGGAATGCCCT
GGCCCTCTCCCGCTCTCAGTTCTGTGTCAGTCTCTTCTACTCCCTGCAAT
CGCTCTCCTACGTCTACCTGGTGATGGAGTACATGGTGGGCGGGGATCTC
AAGTCACTGCTGGCCATGTTTGGGTATTTTGACGAGCCCACGGCCCGGTT
CTACGTGGCCGAGATGGTGATGGCGCTGCAGTATCTGCATCAGCACGGAA
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Gene(s)
(targeted or local)
Allele(s)
Transcripts(s)
Polypeptide(s)
Construct
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PCR template
cDNA
(Japanese National Institute of Genetics, 2012.5.21)
Primer 1
aaggcctacatggccggaccgGGATGTCCACATCGACTACAA
(Japanese National Institute of Genetics, 2012.5.21)
Primer 2
aatctagaggtaccTTCCGTGCTGATGCAGATACT
(Japanese National Institute of Genetics, 2012.5.21)
Comments
hide Collection Information
hide Collection: NIG_RNAi_Fly-1
Symbol NIG_RNAi_Fly-1
Source and Progenitors
Species of derivation D. melanogaster  
Strain of derivation
Vector or progenitor construct
Description
Comprehensive collection of stocks carrying UAS-RNAi transgenes, each designed to target a single protein-coding gene.
(Japanese National Institute of Genetics, 2012.5.21)
Additional data
A collection of over 6,400 RNAi transgenes (over 12,300 lines), each designed to target a single protein-coding gene. Each construct contains a pair of identical 500-bp amplicons produced using cDNA templates. These have been cloned as inverted repeats that are expressed using the binary GAL4/UAS system, producing transcripts capable of assuming a double-stranded RNA structure.
(Japanese National Institute of Genetics, 2012.5.21)
Cell lines
Observed in
Not observed in
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hide Stocks Listed in FlyBase ( 0 )
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hide Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym
7719R
(Japanese National Institute of Genetics, 2012.5.21)
dsRNA-7719R
 
Secondary FlyBase IDs
    hide References ( 1 )
    Stock list
    Japanese National Institute of Genetics, 2012.5.21, RNAi stocks available from NIG-FLY.
    RNAi stocks available from NIG-FLY. [FBrf0218457]